Dideoxy dye terminators

• US 6,949,635 B1
• Filed: 10/26/2000
• Issued: 09/27/2005
• Est. Priority Date: 02/04/1998
• Status: Expired due to Fees

• Current Assignee: GE Healthcare Bio-Sciences Corporation (General Electric Company)
• Original Assignee: Amersham Biosciences Corp. (General Electric Company)
• Inventors: Nampalli, Satyam, Kumar, Shiy, McArdle, Bernard F., Fuller, Carl W.
• Primary Examiner(s): FREDMAN, JEFFREY NORMAN
• Application Number:
  US09/699,030
• Time in Patent Office: 1,797 Days
• Field of Search: 435/6, 435/91.2, 536/23.1, 536/24.3
• US Class Current: 536/23.1
• CPC Class Codes: C07H 19/10 with the saccharide radical esterified by phosphoric or polyphosphoric acids
• CPC Class Codes: C07H 19/20 with the saccharide radical esterified by phosphoric or polyphosphoric acids
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 2525/186 incorporating a non-extendable or blocking moiety

Abstract

A kit for DNA sequencing comprising a first, second, third and fourth dye terminator molecules, each of the dye terminator molecules comprising a dye molecule, a linker of at least 10 atoms in length and either ddATP, ddCTP, ddGTP or ddTTP as a mono or tri-phosphate and a thermostable DNA polymerase.

Claims

1. A compound of the formula (II):

2. A compound of the formula (III):

3. A compound of the formula (IV):

4. A compound of the formula (V):

5. A deoxyribonucleotide sequence containing a compound of formula II, III, IV, or V.

6. A kit for DNA sequencing comprising compounds of formula II, III, IV, and V.

7. The kit of claim 6, further comprising a thermostable DNA polymerase.

8. The kit of claim 7, wherein said polymerase is a thermostable DNA polymerase that has an altered dNMP binding site so as to improve the incorporation of dideoxynucleotides relative to the natural polymerase.