×

Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained

  • US 6,951,719 B1
  • Filed: 11/28/2000
  • Issued: 10/04/2005
  • Est. Priority Date: 08/11/1999
  • Status: Expired due to Term
First Claim
Patent Images

1. A ligase-mediated method of in vitro recombination, comprising:

  • providing oligonucleotide cleavage fragments derived from each of at least two different polynucleotides of a polynucleotide library;

    hybridizing the cleavage fragments to an assembly template;

    ligating together the hybridized fragments that have adjacent ends with a ligase;

    denaturing the ligated fragments from the assembly template; and

    repeating at least the hybridizing, ligating and denaturing steps multiple times to form random recombinant polynucleotides comprised of the ligated fragments;

    wherein said method is performed in the absence of polymerase.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×