Methods and vectors for generating antibodies in avian species and uses therefor
First Claim
1. A process for assessing a proteomics profile of a set of pre-selected DNA sequences isolated from a bio-sample, which comprises:
- 1) cloning each of said DNA sequences into a dual-expression vector that is capable of expressing said DNA sequences in chicken cells, non-chicken cells or in vitro expression systems;
2) delivering said DNA sequence in said dual-expression vector formed in step
1), or protein encoded by said DNA sequence, or a mixture thereof, to a chicken in an amount sufficient to induce detectable production of antibodies to an antigen encoded by said DNA sequence, and recovering said antibodies from said chicken;
3) delivering said DNA sequence, which is delivered to said chicken in step
2), to said non-chicken cells, and recovering proteins or peptides encoded by said DNA sequence from said non-chicken cells, or expressing and recovering proteins or peptides encoded by said DNA sequence in said in vitro expression systems;
4) conducting immunoreactions between said antibodies recovered in step
2) with said proteins or peptides recovered from step
3) to characterize the immunospecificity of said antibodies; and
5) conducting immunoreactions between said antibodies recovered in step
2) with said bio-samples to determine the proteomics profile of said set of pre-selected DNA sequences.
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Abstract
The present invention relates to processes for producing polyclonal and monoclonal antibodies to an antigen in an avian species, preferably in a chicken, using polynucleotide vaccination. The present invention also relates to processes for determining the proteomics profile of a set of pre-selected DNA sequences isolated from a bio-sample, preferably the proteomics profile of a human cDNA library. The present invention additionally relates to processes for identifying physiologically distinguishable markers associated with a physiologically abnormal bio-sample. The present invention further relates to antibody arrays, integrated databases for identification of genes and proteins, multi-functional gene expression vectors, and methods of producing and using such antibody arrays, integrated databases and multi-functional gene expression vectors.
14 Citations
34 Claims
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1. A process for assessing a proteomics profile of a set of pre-selected DNA sequences isolated from a bio-sample, which comprises:
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1) cloning each of said DNA sequences into a dual-expression vector that is capable of expressing said DNA sequences in chicken cells, non-chicken cells or in vitro expression systems;
2) delivering said DNA sequence in said dual-expression vector formed in step
1), or protein encoded by said DNA sequence, or a mixture thereof, to a chicken in an amount sufficient to induce detectable production of antibodies to an antigen encoded by said DNA sequence, and recovering said antibodies from said chicken;
3) delivering said DNA sequence, which is delivered to said chicken in step
2), to said non-chicken cells, and recovering proteins or peptides encoded by said DNA sequence from said non-chicken cells, or expressing and recovering proteins or peptides encoded by said DNA sequence in said in vitro expression systems;
4) conducting immunoreactions between said antibodies recovered in step
2) with said proteins or peptides recovered from step
3) to characterize the immunospecificity of said antibodies; and
5) conducting immunoreactions between said antibodies recovered in step
2) with said bio-samples to determine the proteomics profile of said set of pre-selected DNA sequences. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification