×

Transfection method and uses related thereto

  • US 6,951,757 B2
  • Filed: 03/04/2003
  • Issued: 10/04/2005
  • Est. Priority Date: 09/17/1999
  • Status: Expired due to Term
First Claim
Patent Images

1. A method of introducing nucleic acid molecules into eukaryotic cells, said method comprising the steps of:

  • (a) depositing a nucleic acid molecule-containing mixture onto a surface, wherein said nucleic acid molecule-containing mixture comprises (i) nucleic acid molecules to be introduced into eukaryotic cells and (ii) a gelatin;

    (b) affixing said nucleic acid molecule-containing mixture to said surface; and

    (c) plating eukaryotic cells onto said surface at a density of 0.3×

    105/cm2 to 3.0×

    105/cm2 under appropriate conditions for entry of said nucleic acid molecules into said cells.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×