Spotting device for fabricating microarrays of biological samples and spotting pin inserted therein

• US 6,953,552 B2
• Filed: 04/23/2002
• Issued: 10/11/2005
• Est. Priority Date: 04/23/2001
• Status: Expired due to Fees

• Current Assignee: Bioneer Corporation
• Original Assignee: Bioneer Corporation
• Inventors: Hang-Rae, Kim, Jung-Wook, Ahn, Han-Oh, Park, Gu-Young, Song, Il-Kyu, Choi, Yun-Seok, Cho, Gui-Hwan, Oh
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Gordon, Brian R
• Application Number:
  US10/127,937
• Publication Number:
  US 20020176805A1
• Time in Patent Office: 1,267 Days
• Field of Search: 422/63, 422/99, 422/100, 436/180, 738/633.2, 738/641.7, 738/642.4, 738/643.1, 222/330
• US Class Current: 422/502
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/0255 characterized by the form or material of the pin tip
• CPC Class Codes: B01L 9/00 Supporting devices; Holding devices
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries

Abstract

The present invention relates to a spotting device for fabricating microarrays of biological materials and a spotting pin inserted therein. More particularly, the present invention is directed to a spotting device, which comprises several pins and pin-guiding holder that allows the pins to move accurately and rectilinearly toward up-down direction by guiding and supporting pins at several points, and spotting pins inserted therein.

Claims

1. A spotting device for fabricating microarrays of biological samples, which comprises:

a frame comprising a first side and a second opposing side;

a plurality of spotting pins for spotting biological samples on a plate;

an upper guiding plate which has pin-inserting holes to form at least two (2) pin-supporting points;

a plurality of guide wires each forming at least one (1) pin-supporting point;

a regulation block wherein said guide wires are mounted in a plurality of up-down rows;

wherein said regulation block is inserted in said frame;

a spring plate, inserted in said frame, pushes said regulation block inserted in the frame toward said first side;

a regulation screw, mounted in said frame, pushes said regulation block toward said opposing side; and

a lower guiding plate which has a plurality of pin-inserting holes to form at least two pin-supporting points.

2. The spotting device for fabricating microarrays of biological materials according to claim 1, wherein said guide wire is made of metal.

3. The spotting device for fabricating microarrays of biological materials according to claim 1, wherein said pin is selected from the group consisting of a capillary pin and a stealth pin.

4. The spotting device for fabricating microarrays of biological materials according to claim 3, wherein said capillary pin comprises a capillary part and a reservoir part connected to said capillary part, said reservoir part having larger inner diameter than said capillary part.

5. The spotting device for fabricating microarrays of biological materials according to claim 4, wherein the inner diameter of said capillary part is between 0.03 times to 0.2 times that of the reservoir part.

6. The spotting device for fabricating microarrays of biological materials according to claim 4, wherein the inner diameter of said capillary part is between 0.01 mm to 0.5 mm and the length said capillary is 0.5 mm to 3 mm.

7. The spotting device for fabricating microarrays of biological materials according to claim 1, wherein said guide wire is inserted into respective pairs of holes formed in upper and lower rows on the regulation block.