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Kits employing oligonucleotide-binding e-tag probes

  • US 6,955,874 B2
  • Filed: 04/02/2001
  • Issued: 10/18/2005
  • Est. Priority Date: 04/30/1999
  • Status: Expired due to Fees
First Claim
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1. A kit for detecting the presence or absence of one or more of a plurality nucleotide sequences in a sample, the kit comprising:

  • a capture agent; and

    a plurality of electrophoretic probes selected from the group defined by the formula;


    (D, M)-N-T wherein;

    (D, M)-N is an e-tag reporter released from an electrophoretic probe upon digestion of the electrophoretic probe by a nuclease, the e-tag reporter having a charge;

    D is a detection group;

    M is a mobility modifier consisting of from 1 to 500 atoms selected from the group consisting of carbon, hydrogen, oxygen, phosphorus, nitrogen, sulfur, and boron;

    N is a nucleotide; and

    T is an oligonucleotide specific for at least one of the plurality of nucleotide sequences, each T having a length in the range of from 12 to 60 nucleotides such that at least one nucleotide of T has a capture ligand attached;

    and wherein each e-tag reporter of the plurality of electrophoretic probes has a distinct charge/mass ratio so that the e-tag reporters form distinct peaks in an electropherogram upon electrophoretic separation;

    and wherein the capture ligand specifically binds to the capture agent that has a charge opposite to that of e-tag reporters so that undigested electrophoretic probes are excluded from the electropherogram.

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