Sulfurylase-luciferase fusion proteins and thermostable sulfurylase

• US 6,956,114 B2
• Filed: 04/11/2002
• Issued: 10/18/2005
• Est. Priority Date: 10/30/2001
• Status: Expired due to Fees

• Current Assignee: 454 Life Sciences Corporation (Roche Holding AG)
• Original Assignee: 454 CORPORATION
• Inventors: Srinivasan, Maithreyan, Reifler, Michael
• Primary Examiner(s): Slobodyansky, Elizabeth
• Application Number:
  US10/122,706
• Publication Number:
  US 20030119012A1
• Time in Patent Office: 1,286 Days
• Field of Search: 536/23.2, 536/23.4, 435/194, 435/189, 435/252.3, 435/320.1, 435/325, 435/353, 435/354, 435/366, 435/69.7, 435/254.11, 435/410
• US Class Current: 536/23.2
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12N 9/0069 acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
• CPC Class Codes: C12N 9/1241 Nucleotidyltransferases (2.7.7)

Abstract

The present invention relates to the field of DNA recombinant technology. More specifically, this invention relates to fusion proteins comprising an ATP generating polypeptide joined to a polypeptide that converts ATP into a detectable entity. Accordingly, this invention focuses on sulfurylase-luciferase fusion proteins. This invention also relates to pharmaceutical compositions containing the fusion proteins and methods for using them.

Claims

1. An isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:

NO;

1, 3 and 5.

2. An isolated nucleic acid molecule comprising a nucleic acid sequence of SEQ ID NO:

1.

5. An expression vector comprising the nucleic acid molecule of any one of claims 2-4.

6. A transformed host cell which contains the expression vector of claim 5.

7. The transformed host cell of claim 6 wherein the host cell is a eukaryotic cell.

8. The transformed host cell of claim 7 wherein the eukaryotic cell is a human, rat or mouse cell.

9. The transformed host cell of claim 6 wherein the host cell is a prokaryotic cell.

10. The transformed host cell of claim 9 wherein the prokaryotic cell is a bacterial cell.

3. An isolated nucleic acid molecule comprising a nucleic acid sequence of SEQ ID NO:

3.

4. An isolated nucleic acid molecule comprising a nucleic acid sequence of SEQ ID NO:

5.