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Mobile phase gradient generation microfluidic device

  • US 6,958,119 B2
  • Filed: 02/26/2002
  • Issued: 10/25/2005
  • Est. Priority Date: 02/26/2002
  • Status: Active Grant
First Claim
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1. A microfluidic device for separating the components of a fluid sample, the microfluidic device comprising:

  • a substrate having a microchannel formed in a surface thereof;

    a cover plate arranged over the substrate surface, the cover plate in combination with the microchannel defining a separation conduit for separating the components of the fluid sample according to a specific component property, wherein the separation conduit has an inlet port and an outlet port; and

    an integrated gradient-generation means for generating a gradient of a selected mobile-phase component in a mobile phase by the use of pressure driven flow, including a mechanical switching structure adapted to allow the mobile phase from the gradient-generation means to be transported through the inlet port into the separation conduit and out of the outlet port.

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