Systems and methods for optical actuation of microfluidics based on opto-electrowetting

• US 6,958,132 B2
• Filed: 05/31/2002
• Issued: 10/25/2005
• Est. Priority Date: 05/31/2002
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Wu, Ming C., Chiou, Pei Yu
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Gordon, Brian R.
• Application Number:
  US10/161,220
• Publication Number:
  US 20030224528A1
• Time in Patent Office: 1,243 Days
• Field of Search: 436/180, 436/164, 422 50-104, 204600-602, 204/603
• US Class Current: 422/504
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension
• CPC Class Codes: F04B 19/006 Micropumps F04B43/043 and F04B43/095 take precedence
• CPC Class Codes: G01N 2035/1034 Transferring microquantities of liquid
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

The invention is related to methods and apparatus that manipulate droplets in a microfluidic environment. Advantageously, embodiments of the invention manipulate droplets by controlling the electro-wetting characteristics of a surface with light, thereby inducing a gradient in the surface tension of a droplet. The gradient in the surface tension propels the droplet by capillary force. A variety of operations, such as transporting, joining, cutting, and creating can be performed. Advantageously, embodiments of the invention obviate the need to create a relatively large and complex control electrode array. A plurality of photoconductive cells or a layer of a photoconductive material selectively couples an electrode carrying an electrical bias to otherwise floating conductive cells in response to a beam of light. The electrical bias applied to the conductive cell generates a localized electric field, which can change the contact angle of the droplet, thereby permitting the droplet to be propelled.

Claims

1. A microfluidic circuit adapted to manipulate a droplet in response to light, the microfluidic circuit comprising:

a first wall with at least a conductive layer adapted to be coupled to a first terminal of an alternating current (AC) voltage source;

a second wall adapted to be coupled to a second terminal of the AC voltage source such that an electric field is generated between the first wall and the second wall, where the second wall is responsive to light such that a portion of the second wall that is illuminated by a localized spot of light has a higher electric field intensity than a portion of the second wall that is not illuminated by the localized spot of light, wherein at least one of the first wall or the second wall is further adapted to allow light to pass through;

a cavity defined between the first wall and the second wall, where the cavity is adapted to hold the droplet for manipulation, wherein manipulation includes at least one of transporting, joining, cutting, or creating the droplet during operation; and

wherein the first wall further comprises a hydrophobic inner liner facing the cavity, and wherein the conductive layer of the first wall comprises indium-tin-oxide (ITO) glass and the hydrophobic inner liner comprises polytetrafluoroethytene (PTFE).

2. A microfluidic circuit adapted to manipulate a droplet in response to light, the microfluidic circuit comprising:

a first wall with at least a conductive layer adapted to be coupled to a first terminal of an alternating current (AC) voltage source;

a second wall adapted to be coupled to a second terminal of the AC voltage source such that an electric field is generated between the first wall and the second wall, where the second wall is responsive to light such that a portion of the second wall that is illuminated by a localized spot of light has a higher electric field intensity than a portion of the second wall that is not illuminated by the localized spot of light, wherein at least on of the first wall or the second wall is further adapted to allow light to pass through, wherein the second wall comprises;

a substrate made from an insulating material;

an insulating layer that faces the cavity;

a photoconductive layer disposed between the substrate and the insulating layer;

a first electrode disposed between the substrate and the photoconductive layer, where the first electrode is adapted to be coupled to the second terminal of the AC voltage source; and

a plurality of conductive cells disposed between the photoconductive layer and the insulating layer, wherein the conductive cells are electrically isolated such that the conductive cells are electrically floating; and

a cavity defined between the first wall and the second wall, where the cavity is adapted to hold the droplet for manipulation, wherein manipulation includes at least one of transporting, joining, cutting, or creating the droplet during operation.

3. The microfluidic circuit as defined in claim 2, wherein the insulating layer comprises a layer of silicon oxide (SiO₂) and a layer of polytetrafluoroethylene (PTFE), where the PTFE faces the cavity.

4. The microfluidic circuit as defined in claim 2, wherein the photoconductive layer comprises amorphous silicon.

5. The microfluidic circuit as defined in claim 2, wherein the first electrode comprises conductive strips that extend along a horizontal axis, and wherein the plurality of conductive cells are arranged such that none of the conductive cells are aligned with conductive strips from the first electrode in a vertical direction.

6. The microfluidic circuit as defined in claim 2, wherein the plurality of conductive cells are arranged into rows and columns in a horizontal orientation, and where the first electrode includes horizontal conductors that are disposed between rows of conductive cells in a vertical direction.

7. The microfluidic circuit as defined in claim 2, further comprising a laser assembly adapted to provide a localized spot of light.

8. The microfluidic circuit as defined in claim 2, further comprising a light emitting diode (LED) array adapted to provide a localized spot of light.

9. The microfluidic circuit as defined in claim 2, wherein the second wall is responsive to light of a wavelength that is visible.

10. A microfluidic circuit adapted to manipulate a droplet in response to light, the microfluidic circuit comprising:

a first wall with at least a conductive layer adapted to be coupled to a first terminal of an alternating current (AC) voltage source;

a second wall adapted to be coupled to a second terminal of the AC voltage source such that an electric field is generated between the first wall and the second wall, where the second wall is responsive to light such that a portion of the second wall that is illuminated by a localized spot of light has a higher electric field intensity than, portion of the second wall that is not illuminated by the localized spot of light, wherein at least one of the first wall or the second wall is further adapted to allow light to pass through wherein the second wall comprises;

a substrate made from a transparent insulating material;

an insulating layer that faces the cavity;

a plurality of conductive lines disposed between the substrate and the insulating layer, where the conductive lines are coupled to the second terminal of the AC voltage source;

a plurality of conductive cells disposed between the substrate, and the insulating layer, where the conductive cells are adjacent to the conductive lines; and

a plurality of photoconductive cells, where a photoconductive cell is configured to couple a conductive line to at least one conductive cell; and

a cavity defined between the first wall and the second wall, where the cavity is adapted to hold the droplet for manipulation, wherein manipulation includes at least one of transporting, joining, cuffing, or creating the droplet during operation.

11. The microfluidic circuit as defined in claim 10, wherein the conductive cells are sufficiently transparent to allow light that impinges on a first area of one side of the second wall to pass through and expose a corresponding second area of the first wall.

12. The microfluidic circuit as defined in claim 10, further comprising a layer of a conductive material, wherein the conductive cells and the conductive lines are formed in the layer of the conductive material.

13. The microfluidic circuit as defined in claim 10, where the second wall is responsive to light within 1 second.

14. A microfluidic circuit adapted to manipulate a droplet in response to light, the microfluidic circuit comprising:

a first optical electro-wetting structure adapted to be coupled to a first voltage reference, where the first optical electro-wetting structure includes a first insulating layer and at least a first region sensitive to light such that the first region couples the first voltage reference to at least part of the first insulating layer in response to light;

a second optical electro-wetting structure spaced apart from the first optical electro-wetting structure, where the second optical electro-wetting structure is adapted to be coupled to a second voltage reference, where the second optical electro-wetting structure includes a second insulating layer and a second region sensitive to light such that the second region couples the second voltage reference to at least part of the second insulating layer in response to light, and where the second optical electro-wetting structure is sufficiently transparent to allow light that impinges on a first area of one side of the second optical electro-wetting structure to pass through and expose a corresponding second area of the first optical electro-wetting structure; and

a cavity defined between the first optical electro-wetting structure and the second optical electro-wetting structure, where the cavity is adapted to hold the droplet.

15. The microfluidic circuit as defined in claim 14, wherein the second electro-wetting structure comprises:

a substrate made from a transparent insulating material;

an insulating layer that faces the cavity;

a plurality of conductive lines disposed between the substrate and the insulating layer, where the conductive lines are coupled to the second terminal of the AC voltage source;

a plurality of conductive cells disposed between the substrate and the insulating layer, where the conductive cells are adjacent to the conductive lines; and

a plurality of photoconductive cells, where a photoconductive cell is configured to couple a conductive line to at least one conductive cell.

16. The microfluidic circuit as defined in claim 15, wherein the conductive cells are sufficiently transparent to allow light that impinges on a first area of one side of the second wall to pass through and expose a corresponding second area of the first wall.

17. The microfluidic circuit as defined in claim 15, further comprising a layer of a conductive material, wherein the conductive cells and the conductive lines are formed in the layer of the conductive material.

18. A microfluidic circuit adapted to manipulate a droplet in response to light, the microfluidic circuit comprising:

a first wall with at least a conductive layer adapted to be coupled to a first terminal of an altemating current (AC) voltage source;

a second wall adapted to be coupled to a second terminal of the AC voltage source such that an electric field is generated between the first wall and the second wall, where the second wall is responsive to light such that a portion of the second wall that is illuminated by a localized spot of light has a higher electric field intensity than a portion of the second wall that is not illuminated by the localized spot of light, wherein at least one of the first wall or the second wall is further adapted to allow light to pass through

wherein the second wall further comprises;

a photoconductive layer responsive to light, where the photoconductive layer is adapted to be coupled to the second terminal of the AC voltage source; and

an insulating layer that faces the cavity, where a portion of the insulating layer adjacent to an illuminated portion of the photoconductive layer has a higher electric field intensity than a portion of the insulating layer adjacent to a portion of the photoconductive layer that is not illuminated; and

a cavity defined between the first wall and the second wall, where the cavity is adapted to hold the droplet for manipulation, wherein manipulation includes at least one of transporting, joining, cutting, or creating the droplet during operation.

19. The microfluidic circuit as defined in claim 18, wherein the photoconductive layer comprises amorphous silicon.

20. The microfluidic circuit as defined in claim 18, wherein the insulating layer comprises silicon oxide (SiO2).