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Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products

  • US 6,958,392 B2
  • Filed: 07/18/2001
  • Issued: 10/25/2005
  • Est. Priority Date: 10/09/1998
  • Status: Expired due to Term
First Claim
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1. A method for determining the number of cells of interest in a sample, wherein the cells of interest comprise white blood cells comprising DNA, the method comprising:

  • (a) applying a sample comprising cellular material to a filter, wherein the cellular material comprises cells of interest;

    (b) lysing the cells of interest to form a cell lysate;

    (c) immobilizing the released DNA on or within the filter;

    (d) removing the remaining cell lysate from the filter and retaining the DNA;

    (e) eluting the DNA from the filter;

    (f) quantifying the amount of the DNA eluted from the filter; and

    (g) determining the number of cells of interest in the sample based on the determined quantity of the DNA eluted.

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