Biochannel assay for hybridization with biomaterial
First Claim
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1. A microfluidic device comprising:
- (i) a first microchannel comprising a plurality of spatially distinct regions upon which a member of a specific binding pair is immobilized;
(ii) at least a first entrance port and at least a first exit port for the transportation of at least one test sample;
(iii) a fluid propelling component that controls that flow rate of said test sample; and
(iv) a recirculating arm for recirculating said test sample across a spatially distinct regions.
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Abstract
The invention relates to a microfluidic device with microchannels that have separated regions which have a member of a specific binding pair member such as DNA or RNA bound to porous polymer, beads or structures fabricated into the microchannel. The microchannels of the invention are fabricated from plastic and are operatively associated with a fluid propelling component and detector.
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Citations
24 Claims
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1. A microfluidic device comprising:
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(i) a first microchannel comprising a plurality of spatially distinct regions upon which a member of a specific binding pair is immobilized;
(ii) at least a first entrance port and at least a first exit port for the transportation of at least one test sample;
(iii) a fluid propelling component that controls that flow rate of said test sample; and
(iv) a recirculating arm for recirculating said test sample across a spatially distinct regions. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification