Method and apparatus for analysis and sorting of polynucleotides based on size

• US 6,964,736 B2
• Filed: 12/13/2001
• Issued: 11/15/2005
• Est. Priority Date: 09/25/1996
• Status: Expired due to Fees

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Quake, Stephen, Volkmuth, Wayne D.
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US10/021,850
• Publication Number:
  US 20020053532A1
• Time in Patent Office: 1,433 Days
• Field of Search: 204600-604, 204450-455, 422/81
• US Class Current: 204/452
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/0652 Sorting or classification of particles or molecules
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 2400/0484 Cantilevers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2565/629 being a microfluidic device
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: Y10S 366/03 Micromixers: variable geometry from the pathway influences mixing/agitation of non-laminar fluid flow

Abstract

The invention relates to a microfabricated device and methods of using the device for analyzing and sorting polynucleotide molecules by size.

Claims

1. A microfabricated device for sorting reporter labelled polynucleotide molecules by size, comprising

a chip having a substrate into which is microfabricated at least one analysis unit, said analysis unit comprising

a main channel, having at one end a sample inlet, having along its length a detection region, and having, adjacent and downstream of said detection region, a branch point discrimination region;

a plurality of branch channels originating at the discrimination region and in communication with the main channel;

means for passing a continuous stream of solution containing said molecules through said detection region, such that on average only one molecule occupies the detection region at any given time;

means for measuring the level of reporter from each molecule within the detection region; and

means for directing said molecule to a selected branch channel based on said level of reporter.

2. The device of claim 1, wherein said directing means comprises a pair of electrodes effective to apply an electric field across the discrimination region, said field being effective to direct a particular molecule into a selected branch channel.

3. The device of claim 1, wherein a flow of molecules is maintained through the device via a pump or pressure differential, and said directing means comprises a valve structure at said branch point effective to permit said molecule to enter only one of said branch channels.

4. The device of claim 1, wherein a flow of molecules is maintained through the device via a pump or pressure differential, and said directing means comprises, for each branch channel, a valve structure downstream of said branch point effective to allow or curtail flow through said channel.

5. The device of claim 1, wherein a flow of molecules is maintained through the device via a pump or pressure differential, and said directing means comprises, for each branch channel, a pressure adjusting means at the outlet of each branch channel effective to allow or curtail flow through said channel.

6. The device of claim 1, wherein said channels are between about 1 and 10 μ

m in width and between about 1 and 10 μ

m in depth.

7. The device of claim 1, wherein said detection region has a volume of between about 1 fl and 1 pl.

8. The device of claim 1, further comprising a glass cover slip bonded to said substrate and covering said channels.

9. The device of claim 1, wherein said exciting and measuring means comprises a fluorescence microscope holding said device.

10. The device of claim 1, wherein said exciting means comprises an external or integrated semiconductor laser, and said measuring means comprises an integrated photodiode.

11. A method of isolating polynucleotides having a selected size, comprising

A) flowing a continuous stream of solution containing reporter-labeled polynucleotides through a channel comprising a detection region having a selected volume, where the concentration of the molecules in the solution is such that most molecules pass through the detection region one by one,

B) determining the size of each molecule as it passes through the detection region by measuring the level of said reporter,

C) in said continuous stream of solution, diverting (i) molecules having said selected size into a first branch channel, and (ii) molecules not having said selected size into a second branch channel, and

D) collecting polynucleotides diverted into said first branch channel.

12. The method of claim 11, wherein the concentration of polynucleotides in said solution is between about 100 fM and about 100 pM.

13. The method of claim 11, wherein said detection region volume is between about 1 fl and about 1 pl.

14. The method of claim 11, wherein said determining includes quantitating an optical signal from an optical reporter associated with said polynucleotides.

15. The method of claim 14, wherein said optical signal is a fluorescence signal and said optical reporter includes a fluorescent moiety.

16. The method of claim 15, wherein said optical reporter is selected from the group consisting of POPO, BOBO, YOYO, and TOTO.

17. The method of claim 11, wherein said diverting includes the transient application of an electric field effective to bias (i) a molecule having said selected size to enter said first branch channel, and (ii) a molecule not having said selected size to enter said second branch channel.

18. The method of claim 11, for diverting a molecule having said selected size into said first branch channel, wherein said diverting includes blocking the flow in said second branch channel such that said continuous stream of solution carries the molecule having said selected size into said first branch channel.

19. The method of claim 11, for diverting a molecule not having said selected size into said second branch channel, wherein said diverting includes blocking the flow in said first branch channel such that said continuous stream of solution carries the fragment not having said selected size into said second branch channel.

20. The method of claim 11, wherein said diverting includes a mechanical switch effective to direct (i) a fragment having said selected size to enter said first branch channel, and (ii) a fragment not having said selected size to enter said second branch channel.

21. The method of claim 11, wherein said selected size is between about 100 bp and about 10 mb.

22. The method of claim 21 wherein said selected size is between about 100 bp and about 100 kb.

23. The method of claim 22 wherein said selected size is between about 500 bp and about 50 kb.