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Pyrimidine derivatives for labeled binding partners

  • US 6,967,079 B2
  • Filed: 11/14/2002
  • Issued: 11/22/2005
  • Est. Priority Date: 09/17/1993
  • Status: Expired due to Fees
First Claim
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1. A primer for amplification or detection of a nucleic acid, the primer comprising a compound of the formula Bx-R1, a tautomer, solvate or salt thereof, wherein R1 is a binding partner and Bx is of the formula:

  • embedded imagewherein a and b are 0 or 1, provided that the total of a and b is 0 or 1;

    A is N or C;

    X is S, O, —

    C(O)—

    , NH or NCH2R6;

    Y is —

    C(O)—

    ;

    Z is taken together with A to form an aryl or heteroaryl ring structure comprising 5 or 6 ring atoms wherein the heteroaryl ring comprises a single O ring heteroatom, a single N ring heteroatom, a single S ring heteroatom, a single O and a single N ring heteroatom separated by a carbon atom, a single S and a single N ring heteroatom separated by a carbon atom, 2 N ring heteroatoms separated by a carbon atom, or 3 N ring heteroatoms at least two of which are separated by a carbon atom, and wherein the aryl or heteroaryl ring carbon atoms are unsubstituted with other than H or at least 1 nonbridging ring carbon atom is substituted with R6 or ═

    O, R6 is independently H, C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, NO2, N(R3)2, CN or halo, or R6 taken together with an adjacent R6 to complete a ring containing 5 to 6 ring atoms, and tautomers, solvates and salts thereof; and

    R3 is a protecting group or H;

    provided that where a is 0, b is 1 and R1 is embedded imagein which D2 is independently hydroxyl, blocked hydroxyl, mono-, di- or triphosphate, or an oligodeoxyribonucleotide otherwise containing only the bases A, G, T and C; and

    D3 is H or OH, then Z, taken together with A, is not unsubstituted phenyl.

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