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Method for determining the presence or absence of respiring cells on a three-dimensional scaffold

  • US 6,967,086 B2
  • Filed: 03/28/2002
  • Issued: 11/22/2005
  • Est. Priority Date: 04/18/1991
  • Status: Expired due to Term
First Claim
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1. A method for determining presence or absence of respiring cells comprising:

  • (i) depositing a three-dimensional biomimetic scaffold and cells onto a sensor composition, said sensor composition comprising a luminescent compound that exhibits a change in luminescent property when irradiated with light containing wavelengths which cause said compound to luminesce upon exposure to oxygen;

    (ii) irradiating said sensor composition with light to cause luminescence;

    (iii) determining the resultant luminescent light intensity emitted;

    (iv) determining, based on said emitted luminescent light intensity, the presence or absence of respiring cells.

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