Method for determining the presence or absence of respiring cells on a three-dimensional scaffold
First Claim
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1. A method for determining presence or absence of respiring cells comprising:
- (i) depositing a three-dimensional biomimetic scaffold and cells onto a sensor composition, said sensor composition comprising a luminescent compound that exhibits a change in luminescent property when irradiated with light containing wavelengths which cause said compound to luminesce upon exposure to oxygen;
(ii) irradiating said sensor composition with light to cause luminescence;
(iii) determining the resultant luminescent light intensity emitted;
(iv) determining, based on said emitted luminescent light intensity, the presence or absence of respiring cells.
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Abstract
The invention provides a method and apparatus for determining the presence or absence of respiring cells, involving combining a three-dimensional biomimetic scaffold and cells onto a sensor composition.
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Citations
21 Claims
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1. A method for determining presence or absence of respiring cells comprising:
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(i) depositing a three-dimensional biomimetic scaffold and cells onto a sensor composition, said sensor composition comprising a luminescent compound that exhibits a change in luminescent property when irradiated with light containing wavelengths which cause said compound to luminesce upon exposure to oxygen;
(ii) irradiating said sensor composition with light to cause luminescence;
(iii) determining the resultant luminescent light intensity emitted;
(iv) determining, based on said emitted luminescent light intensity, the presence or absence of respiring cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification