Methods for genomic analysis
First Claim
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1. A method for selecting SNP haplotype patterns, comprising:
- (a) isolating a set of substantially identical nucleic acid strands from a plurality of different origins for analysis;
(b) determining more than one SNP location in each nucleic acid strand in said set;
(c) identifying SNP locations that are linked in said nucleic acid, wherein said SNP locations that are linked form a SNP haplotype block, thereby identifying said SNP haplotype block;
(d) identifying isolate SNP haplotype blocks;
(e) identifying SNP haplotype patterns that occur in the SNP haplotype block identified in step (c) and the isolate SNP haplotype blocks identified in step (d); and
(f) selecting each identified SNP haplotype pattern that occurs in at least two of said substantially identical nucleic acid strands in said set.
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Abstract
The present invention relates to methods for identifying variations that occur in the human genome and relating these variations to the genetic basis of disease and drug response. In particular, the present invention relates to identifying individual SNPs, determining SNP haplotype blocks and patterns, and, further, using the SNP haplotype blocks and patterns to dissect the genetic bases of disease and drug response. The methods of the present invention are useful in whole genome analysis.
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13 Claims
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1. A method for selecting SNP haplotype patterns, comprising:
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(a) isolating a set of substantially identical nucleic acid strands from a plurality of different origins for analysis;
(b) determining more than one SNP location in each nucleic acid strand in said set;
(c) identifying SNP locations that are linked in said nucleic acid, wherein said SNP locations that are linked form a SNP haplotype block, thereby identifying said SNP haplotype block;
(d) identifying isolate SNP haplotype blocks;
(e) identifying SNP haplotype patterns that occur in the SNP haplotype block identified in step (c) and the isolate SNP haplotype blocks identified in step (d); and
(f) selecting each identified SNP haplotype pattern that occurs in at least two of said substantially identical nucleic acid strands in said set. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for selecting a SNP haplotype block useful in genomic analysis, comprising:
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isolating a set of substantially identical DNA strands from at least about five different origins for analysis;
analyzing at least about 1×
106 bases from each of said substantially identical DNA strands in said set;
determining more than one SNP location in each of said substantially identical DNA strands in said set;
identifying SNP locations that are linked in said set of substantially identical DNA strands, wherein said SNP locations that are linked form a SNP haplotype block;
identifying SNP haplotype patterns that occur in said SNP haplotype block; and
selecting each identified SNP haplotype pattern that occurs in any of said substantially identical DNA strands in said set.
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Specification