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Method and apparatus for detecting fluorescence of a sample

  • US 6,970,246 B2
  • Filed: 04/11/2001
  • Issued: 11/29/2005
  • Est. Priority Date: 04/11/2000
  • Status: Active Grant
First Claim
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1. An apparatus for detecting the fluorescence of a sample comprising:

  • a first excitation light system including at least a first light source, the first light source having a main light path;

    a sample plane for positioning said sample;

    a detection device including at least a first detector for detecting fluorescence signals from the sample, an axis between the detection device and the sample plane being a detection-sample axis;

    a processor coupled to receive data from the detector;

    a focusing device for focusing the signals onto the detection device, said focusing device having a collection angle, an angle between the main light path of the first light source and the detection-sample axis being between the collection angle/2 and 90°

    , and the focusing device providing a magnification in a range of 2/1 to 1/10.

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