Materials and methods for nerve grafting, selection of nerve grafts, and in vitro nerve tissue culture
First Claim
Patent Images
1. A method for promoting repair of a damaged nerve, the method comprising:
- culturing a nerve graft in vitro under predegenerating conditions that remodel the nerve graft and that increase the neurite-promoting activity of the nerve graft when subsequently implanted;
rendering the nerve graft acellular by killing cells in the nerve graft; and
implanting the nerve graft at a site of damage.
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Abstract
The subject invention pertains to compositions and methods for culturing nerve tissue in vitro and nerve grafts produced using such methods. The compositions and methods of the subject invention can be employed to restore the continuity of nerve interrupted by disease, traumatic events or surgical procedures. The invention also concerns methods for promoting repair of damaged nerve tissue using the present compositions and nerve tissue treated according to such methods.
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Citations
46 Claims
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1. A method for promoting repair of a damaged nerve, the method comprising:
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culturing a nerve graft in vitro under predegenerating conditions that remodel the nerve graft and that increase the neurite-promoting activity of the nerve graft when subsequently implanted; rendering the nerve graft acellular by killing cells in the nerve graft; and implanting the nerve graft at a site of damage. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A method for promoting repair of a damaged nerve, the method comprising:
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culturing a nerve graft in vitro under predegenerating conditions that increase the neurite-promoting activity of the nerve graft when subsequently implanted, the conditions comprising a temperature within the range of about 10°
C. for a period of time within the range of about 24 hours to about 96 hours;rendering the nerve graft acellular by killing cells in the nerve graft; and implanting the nerve graft at a site of damage. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45)
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46. A method for promoting repair of a damaged nerve, the method comprising:
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placing a nerve graft in conditions in vitro that are permissive to the activation and proliferation of cells within the nerve graft, are permissive to the activity of enzymes produced by cells within the nerve graft, are permissive to the degeneration and remodeling of the nerve graft in vitro, and increase the neurite-promoting activity of the nerve graft when subsequently implanted; rendering the nerve graft acellular by killing cells in the nerve graft; and implanting the nerve graft at a site of damage.
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Specification