×

Methods to increase nucleotide signals by raman scattering

  • US 6,972,173 B2
  • Filed: 03/14/2002
  • Issued: 12/06/2005
  • Est. Priority Date: 03/14/2002
  • Status: Active Grant
First Claim
Patent Images

1. A method comprising:

  • a) obtaining nucleotides covalently linked to gold or silver, or gold and silver, nanoparticle(s), wherein the nucleotide and nanoparticle are linked via a terminal highly reactive cross-linking group selected from the group consisting of epoxide groups, azido groups, triazine groups, arylazido groups, and diazo groups;

    b) synthesizing a nucleic acid comprising the labeled nucleotides;

    c) immobilizing the nucleic acid of b) on a solid substrate;

    d) sequentially releasing nucleotides from one end of the nucleic acid;

    e) separating the released nucleotides from the immobilized nucleic acid by transferring the nucleotides through a microfluidic channel;

    f) identifying nucleotides by Raman spectroscopy; and

    g) determining the sequence of the nucleic acid.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×