Use of polyacrylamide gel for forming a connective-tissue capsule in a mammal for cultivating allogenic and xenogenic cells

• US 6,972,194 B1
• Filed: 11/22/2000
• Issued: 12/06/2005
• Est. Priority Date: 12/08/1999
• Status: Expired due to Fees

• Current Assignee: Vercell Biotechnology BV
• Original Assignee: Vercell Biotechnology BV
• Inventors: Severin, Sergei Evgenievich, Kotelevits, Alexei Gennadievich, Zybin, Dmitry Vladimirovich, Mironova, Ljubov Leonidovna, Sologub, Vladimir Konstantinovich
• Primary Examiner(s): Canella, Karen A.
• Application Number:
  US09/890,496
• Time in Patent Office: 1,840 Days
• Field of Search: 435366-371, 435/373, 435/374, 435/375, 435/382
• US Class Current: 435/382
• CPC Class Codes: A61K 2035/126 Immunoprotecting barriers, e.g. jackets, diffusion chambers
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 47/32 Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers , poly(meth)acrylates, or polyvinyl pyrrolidone
• CPC Class Codes: A61K 9/0019 Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner non-active ingredients are additionally classified in A61K47/00
• CPC Class Codes: A61K 9/0024 Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L
• CPC Class Codes: A61P 15/10 for impotence
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 5/50 for increasing or potentiating the activity of insulin
• CPC Class Codes: C12N 2502/243 Cells of the female genital tract, non-germinal ovarian cells
• CPC Class Codes: C12N 2502/30 tumour cells
• CPC Class Codes: C12N 5/0677 Three-dimensional culture, tissue culture or organ culture; Encapsulated cells

Abstract

The invention relates to the field of medicine and more particularly it relates to the problem of vaccination against tumor cells and vaccinotherapy of oncological diseases, and also to a method of treating diabetes mellitus. In the invention a new method of cultivating cells is proposed, which contemplates forming a capsule of a polyacrylamide gel in the tissue of an animal, including a human, into which capsule desirable cells are injected. The invention provides for maintaining the viability of cells during a long period of time.

Claims

1. A method of forming in a mammal a connective tissue capsule for maintaining transplanted allogenic or xenogenic cells comprising introducing a polyacrylamide gel into a tissue of the mammal so as to cause the connective tissue capsule to form around the polyacrylamide gel, wherein the mammal suffers from a pathology and the method comprises introducing into and maintaining in said connective tissue capsule transplanted allogenic or xenogenic cells that aid in treating the pathology.

2. A method according to claim 1, wherein the mammal is a human.

3. A method according to claim 1, wherein the pathology is diabetes mellitus.

4. A method according to claim 1, wherein pancreatic B-cells are introduced into and maintained in said connective tissue capsule.

5. A method according to claim 4, wherein the pancreatic β

-cells are cells from rabbits or pigs.

6. A method according to claim 1, wherein the connective tissue capsule is formed by subcutaneous injection of the polyacrylamide gel into the mammal.

7. A method of introducing allogenic or xenogenic cells into a mammal, comprising introducing a polyacrylamide gel into a mammal, thereby inducing formation of a connective tissue capsule around said gel, and thereafter, injecting allogenic or xenogenic cells of a mammal into said connective tissue capsule.

8. A method according to claim 7, wherein the gel is introduced by subcutaneous injection.

9. A method according to claim 7, wherein said allogenic or xenogenic cells are tumor cells.

10. A method according to claim 7, wherein said allogenic or xenogenic cells are Leydig'"'"'s cells.

16. A method according to claim 7, wherein said transplanted cells are injected into said capsule such that they persist in the mammal for a period that exceeds a period that said transplanted cells would persist without prior formation of the connective tissue capsule.

11. A method of treating a pathology in a mammal, comprising introducing a polyacrylamide gel into a mammal, thereby inducing formation of a connective tissue capsule around said gel;

and thereafter transplanting allogenic or xenogenic cells of a mammal into said connective tissue capsule, said cells being maintained in said capsule and producing a biologically active substance which is released from said capsule for treatment of the pathology.

12. A method according to claim 11, wherein said pathology is diabetes melitus, said transplanted cells are pancreatic β

-cells, and said biologically active substance is insulin.

13. A method according to claim 12, wherein said β

-cells are from rabbits or pigs.

17. A method according to claim 11, wherein said transplanted cells persist in the mammal for a period of time that exceeds a period that said transplanted cells would persist without prior formation of the connective tissue capsule.

14. A method of forming in a mammal a connective tissue capsule for maintaining transplanted allogenic or xenogenic cells comprising introducing a polyacrylamide gel into a tissue of the mammal so as to cause the connective tissue capsule to form around the polyacrylamide gel further comprising transplanting allogenic or xenogenic cells into the connective tissue capsule so as to maintain the cells in the capsule for a period of time.

15. A method according to claim 14, wherein said period of time exceeds a period that said transplanted cells would persist in the mammal without prior formation of said connective tissue capsule.