Supported lattice for cell cultivation

• US 6,974,586 B2
• Filed: 10/31/2001
• Issued: 12/13/2005
• Est. Priority Date: 10/31/2000
• Status: Active Grant

• Current Assignee: The Secant Group, LLC (Compagnie Generale Des Etablissements Michelin Sca)
• Original Assignee: SECANT MEDICAL, LLC (Compagnie Generale DES Etablissements Michelin SCA)
• Inventors: Kaufmann, Carol A., Greenhalgh, E. Skott
• Primary Examiner(s): Naff, David M.
• Application Number:
  US09/998,880
• Publication Number:
  US 20020066360A1
• Time in Patent Office: 1,504 Days
• Field of Search: 424/423, 424/93.7, 435/174, 435/177, 435/180, 435/395
• US Class Current: 424/423
• CPC Class Codes: A61B 17/1128 of nerves
• CPC Class Codes: A61F 2/0063 Implantable repair or support meshes, e.g. hernia meshes
• CPC Class Codes: A61F 2/06 Blood vessels
• CPC Class Codes: A61F 2/10 Hair or skin implants
• CPC Class Codes: A61F 2002/0086 for preferentially controlling or promoting the growth of specific types of cells or tissues of bone tissue onto implantable joints A61F2002/3093
• CPC Class Codes: C12N 2533/10 Mineral substrates
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 2533/40 Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

A supported lattice is disclosed having a support substrate formed of a plurality of resilient filamentary members braided together to yield a coarse mesh having relatively large interstices and a cell cultivation lattice formed of a plurality of flexible filamentary members braided together and with the resilient filamentary members to form a fine mesh having small interstices. The cell cultivation lattice provides a bed adapted for growing cells in a two-dimensional array across the large interstices of the support substrate to form a continuous surface of living tissue useful to form a graft.

Claims

1. A supported lattice for cultivating living cells to form living tissue, said supported lattice comprising:

a first support substrate comprising a first elongated tube formed of a plurality of first resilient filamentary members interlaced together, said first resilient filamentary members being interlaced to form a first mesh defined by interstices that are large compared with said living cells such that said cells do not bridge said interstices;

a first cell cultivation lattice comprising a plurality of first flexible filamentary members interlaced with one another and said first resilient filamentary members, said first flexible filamentary members being interlaced to form a second mesh defined by interstices smaller than the interstices formed by the first resilient filamentary members and adapted for growing cells in a two-dimensional array across said large interstices of said first support substrate to form a first substantially continuous surface comprising said living tissues;

said supported lattice further comprising a second elongated tube positioned coaxially within said first elongated tube, said second elongated tube comprising;

a second support substrate comprising a plurality of second resilient filamentary members interlaced together, said second resilient filamentary members being interlaced to form a third mesh defined by interstices that are large compared with said living cells such that said cells do not bridge said interstices; and

a second cell cultivation lattice comprising a plurality of second flexible filamentary members interlaced with one another and said second resilient filamentary members, said second flexible filamentary members being interlaced to form a fourth mesh defined by interstices smaller than the interstices formed by the second resilient filamentary members and adapted for growing cells in a two-dimensional array across said large interstices of said second support substrate to form a second substantially continuous surface comprising said living tissue.

2. A supported lattice according to claim 1, wherein said resilient and said flexible filamentary members are interlaced by braiding.

3. A supported lattice according to claim 1, wherein said interstices between said first flexible filamentary members have an average size between about 60 microns and about 80 microns.

4. A supported lattice according to claim 1, wherein said first resilient filamentary members comprise monofilaments selected from the group consisting of stainless steel, nitinol and elgiloy monofilaments.

5. A supported lattice according to claim 4, wherein said first flexible filamentary members comprise multi-filament yarns.

6. A supported lattice according to claim 5, wherein said multi-filament yarns are elastic.

7. A supported lattice according to claim 6, wherein said multi-filament yarns comprise textured yarns.

9. A supported lattice according to claim 1, wherein said interstices of said first cell cultivation lattice have an average size between about 120 microns and about 150 microns and said interstices of said second cell cultivation lattice have an average size between about 60 microns and about 80 microns.

10. A supported lattice according to claim 1, further comprising a plurality of elongated filamentary bonding members interlaced with said resilient filamentary members comprising one of said elongated tubes, said filamentary bonding members having a relatively low melting point and being heat fused to said resilient filamentary members comprising said tubes, thereby joining said tubes to one another.

11. A supported lattice according to claim 1, wherein said first resilient and said first flexible filamentary members comprise bio-absorbable material selected from the group consisting of polylactic acid, polyglycolic acid and hydroxyacetic acid, said first resilient filamentary members having a relatively larger denier than said first flexible filamentary members.

12. A supported lattice according to claim 2, wherein said first resilient filamentary members comprise monofilaments selected from the group consisting of stainless steel, nitinol and elgiloy monofilaments.

13. A supported lattice according to claim 12, wherein said first flexible filamentary members comprise multi-filament yarns.

14. A supported lattice according to claim 2, wherein said first resilient filamentary members comprise a heat shrinkable material having a relatively low melting point and said first flexible filamentary members comprise multi-filament yarns of a dimensionally stable material having a relatively higher melting point, said multi-filament yarns being in a buckled configuration inwardly and outwardly from said first support substrate thereby forming dimples in said lattice, said dimples being located within said relatively large interstices between said first resilient filamentary members, said first resilient filamentary members being heat fused to one another and to said first flexible filamentary members at points of mutual contact, thereby locking said first flexible filamentary members in said buckled configuration.

15. A supported lattice according to claim 14, wherein filaments comprising said multi-filament yarns assume said buckled configuration independently of one another thereby forming relatively small interstices between said filaments.

16. A supported lattice according to claim 14, wherein said first resilient filamentary members comprise polypropylene and said first flexible filamentary members comprise polytetrafluoroethylene.

8. A supported lattice according to claim 8, wherein said multi-filament yarns are selected from the group consisting of polyester, polytetrafluoroethylene, polypropylene and polyethylene.