Therapeutic polypeptides, nucleic acids encoding same, and methods of use

• US 6,974,684 B2
• Filed: 08/01/2002
• Issued: 12/13/2005
• Est. Priority Date: 08/08/2001
• Status: Expired due to Fees

• Current Assignee: CuraGen Corporation (Celldex Therapeutics Incorporated)
• Original Assignee: CuraGen Corporation (Celldex Therapeutics Incorporated)
• Inventors: Guo, Xiaojia Sasha, Anderson, David W., Shimkets, Richard A., Khramtsov, Nikolai, Pena, Carol E. A., Rieger, Daniel K., Zhong, Mei, Lepley, Denise M., Alsobrook, John P. II, Boldog, Ferenc L., Gorman, Linda, Patturajan, Meera, Smithson, Glennda, Gangolli, Esha A., Peyman, John A., Edinger, Shlomit R., Vernet, Corine A. M., Voss, Edward Z., Spytek, Kimberly A., Burgess, Catherine E., Casman, Stacie J., MacDougall, John R.
• Primary Examiner(s): Weber, Jon
• Assistant Examiner(s): Mondesi, Robert B.
• Application Number:
  US10/211,689
• Publication Number:
  US 20030232347A1
• Time in Patent Office: 1,230 Days
• Field of Search: 530/350, 435/7.1, 435/6, 435/320.1, 435/325, 435/69.1, 435/7, 514/2, 536/23.1
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748

Abstract

Disclosed herein are nucleic acid sequences that encode G-coupled protein-receptor related polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of disorders involving any one of these novel human nucleic acids and proteins.

Claims

1. An isolated nucleic acid molecule comprising an open reading frame encoding an amino acid sequence of SEQ ID NO:

10.

2. The nucleic acid molecule of claim 1, wherein the nucleic acid molecule is naturally occurring.

3. The isolated nucleic acid molecule of claim 1, comprising nucleotides 15-636 of SEQ ID NO:

9 or nucleotides 14-634 of SEQ ID NO;

11.

4. An isolated nucleic acid molecule, wherein said nucleic acid molecule hybridizes under stringent conditions to the nucleotide sequence of claim 3, or a complement of said nucleotide sequence, and wherein said stringent conditions comprise 6×

SSC, 50 mM Tris HCl (pH 7.5), 1 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.02% BSA, and 500 mg/ml denatured salmon sperm DNA at 65°

C. followed by one or more washes in 0.2×

SSC, 0.0 1% BSA at 50°

C.

5. A vector comprising the nucleic acid molecule of claim 1.

6. The vector of claim 5, further comprising a promoter operably linked to said nucleic acid molecule.

7. A cell comprising the vector of claim 5.

8. A method of producing a polypeptide comprising an amino acid sequence of SEQ ID NO:

10 comprising culturing a cell under conditions that lead to expression of the polypeptide, wherein said cell comprises the vector of claim 5 or 6.

9. The method of claim 8 wherein the cell is a bacterial cell.

10. The method of claim 8 wherein the cell is an insect cell.

11. The method of claim 8 wherein the cell is a yeast cell.

12. The method of claim 8 wherein the cell is a mammalian cell.

13. An isolated nucleic acid molecule comprising an open reading frame encoding amino acids 16-207 of SEQ ID NO:

10.

14. The isolated nucleic acid molecule of claim 13 comprising nucleotides 59-635 of SEQ ID NO:

9 or nucleotides 58-634 of SEQ ID NO;

11.

15. A vector comprising the nucleic acid molecule of claim 13.

16. A cell comprising the vector of claim 15.

17. A method of producing a polypeptide comprising amino acids 16-207 of SEQ ID NO:

10, said method comprising culturing a cell under conditions that lead to expression of the polypeptide, wherein said cell comprises a vector of claim 15.