Methods and systems for performing multiple reactions by interfacial mixing
First Claim
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1. A method of analyzing a plurality of reactions, comprising:
- serially introducing plugs of first, second and third fluid borne reagents into a first fluid conduit under conditions suitable for performing the plurality of reactions whereby the plug of the first fluid borne reagent is abutted by the plug of the second fluid borne reagent at a first interface, and the plug of the second fluid borne reagent is abutted by the plug of the third fluid borne reagent at a second interface;
allowing sufficient time for diffusion of effective amounts of the first and second reagents across the first interface whereupon the first and second reagents mix and react in a first reaction;
allowing sufficient time for diffusion of effective amounts of the second and third reagents across the second interface, whereupon the second and third reagents react in a second reaction; and
analyzing results of the first and second reactions.
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Abstract
Methods and systems of performing multiple reactions in a high throughput format by utilizing interfacial mixing of adjacently positioned reagent slugs in a fluid conduit. Preferred applications of the methods and systems are in performing biochemical analyses, including genotyping experiments for multiple different loci on multiple different patient samples. Microfluidic systems are provided that increase throughput, automation and integration of the overall reactions to be carried out.
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Citations
11 Claims
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1. A method of analyzing a plurality of reactions, comprising:
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serially introducing plugs of first, second and third fluid borne reagents into a first fluid conduit under conditions suitable for performing the plurality of reactions whereby the plug of the first fluid borne reagent is abutted by the plug of the second fluid borne reagent at a first interface, and the plug of the second fluid borne reagent is abutted by the plug of the third fluid borne reagent at a second interface; allowing sufficient time for diffusion of effective amounts of the first and second reagents across the first interface whereupon the first and second reagents mix and react in a first reaction; allowing sufficient time for diffusion of effective amounts of the second and third reagents across the second interface, whereupon the second and third reagents react in a second reaction; and analyzing results of the first and second reactions. - View Dependent Claims (2, 3)
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4. A method of performing a plurality of reactions, comprising:
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introducing a first volume of a first reagent into a fluid channel; introducing a first volume of a second reagent into the first fluid channel, the first volume of the second reagent abutting the first volume of the first reagent; introducing a first volume of a third reagent into the first fluid channel, the first volume of the third reagent abutting the first volume of the second reagent; diffusing the first and second reagents to diffuse together to form a first reaction in the first fluid channel; diffusing the second reagent and the third reagent together in the first fluid channel to form a second reaction mixture; and separately detecting a first reaction product in the first reaction mixture and a second reaction product in the second reaction mixture. - View Dependent Claims (5, 6, 7, 8, 9, 10, 11)
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Specification