Methods for ultrasonic imaging and treating diseased tissues

• US 6,979,296 B2
• Filed: 04/03/2003
• Issued: 12/27/2005
• Est. Priority Date: 04/03/2002
• Status: Expired due to Fees

• Current Assignee: SBM Biologics, Inc.
• Original Assignee: SBM Biologics, Inc.
• Inventors: See, Jacko R.
• Primary Examiner(s): IMAM, ALI M
• Application Number:
  US10/406,748
• Publication Number:
  US 20040030252A1
• Time in Patent Office: 999 Days
• Field of Search: 600/439, 600459-472, 600/458, 424/9.51, 424/9.92, 424/85.1
• US Class Current: 600/458
• CPC Class Codes: A61K 49/227 Liposomes, lipoprotein vesicles, e.g. LDL or HDL lipoproteins, micelles, e.g. phospholipidic or polymeric
• CPC Class Codes: A61P 25/28 for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/10 for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis

Abstract

A method for imaging diseased tissue is provided. The method provides delivering to the diseased tissue a liposomal imaging agent comprising liposomes containing an imaging agent. The liposomal imaging agent is bound to an antibody by an antigenic linker so that the antibody binds the liposomal imaging agent to the diseased tissue. The liposomes are broken to release the imaging agent using a catheter, and the imaging agent is viewed using an imaging technique. The tissue can also be treated by delivering a therapeutic agent to the tissue using the catheter, for example, within the liposomes of the liposomal imaging agent.

Claims

1. A method for imaging diseased tissue, comprising:

delivering to the diseased tissue a liposomal imaging agent comprising liposomes containing an imaging agent, the liposomal imaging agent being bound to an antibody by an antigenic linker, wherein the antibody binds the liposomal imaging agent to the diseased tissue;

breaking the liposomes to release the imaging agent using a catheter; and

viewing the imaging agent using an imaging technique.

2. The method according to claim 1, wherein the liposomal imaging agent is delivered to the diseased tissue using the catheter.

3. The method according to claim 1, wherein the liposomes are broken by applying radio frequency energy or ultrasound energy with the catheter.

4. The method according to claim 1, wherein the antibody is selected from the group consisting of polyclonal antibodies, monoclonal antibodies, cytokines, growth factors, immunoglobulin, matrix metalloproteases, amino acids, peptides, extracellular matrix proteins, purified neurochemicals, chemokines, RNA fragments, DNA fragments, and nucleic acids.

5. The method according to claim 1, wherein the liposomal imaging agent comprises gas-filled liposomes.

6. The method according to claim 5, wherein the gas-filled liposomes comprise at least one gas selected from the group consisting of air, oxygen, hydrogen, nitrogen, fluorocarbons, argon, xenon, helium, and fluoropropanes.

7. The method according to claim 5, wherein the gas-filled liposomes are prepared by:

preparing liposomes comprising an inner retentate surrounded by one or more outer layers;

freezing and thawing the liquid liposomes to induce the inner retentate to go directly from the solid state to the gaseous state while the one or more outer layers of the liposome do not.

8. The method according to claim 1, further comprising delivering energy directly into the diseased tissue using the catheter.

9. The method according to claim 8, wherein the energy is selected from radio frequency energy and ultrasound energy.

10. The method according to claim 1, further comprising, after viewing the imaging agent, breaking the antigenic linkage using the catheter to release the liposomes from the diseased tissue.

11. A method for imaging and treating diseased tissue comprising:

imaging diseased tissue according to the method of claim 1, wherein the liposomes containing the imaging agent further contain a therapeutic agent; and

breaking the liposomes to release the therapeutic agent to thereby treat the diseased tissue with the therapeutic agent.

12. The method according to claim 11, wherein the therapeutic agent is selected from the group consisting of trastuzumab, infliximab and abciximab.

13. A method for imaging and treating diseased tissue comprising:

imaging diseased tissue according to the method of claim 1; and

introducing a therapeutic agent to the diseased tissue using the catheter to thereby treat the diseased tissue.

14. The method according to claim 13, wherein the therapeutic agent is selected from the group consisting of trastuzumab, infliximab and abciximab.

15. The method according to claim 13, wherein the therapeutic agent is contained within liposomes.

16. The method according to claim 1, wherein the diseased tissue is selected from the group consisting of tumors, areas of infection, areas of infarction, areas of scarring, areas of degeneration, and areas of tissue overgrowth of unknown etiology.