80090, human fucosyltransferase nucleic acid molecules and uses thereof

• US 6,979,564 B2
• Filed: 10/19/2001
• Issued: 12/27/2005
• Est. Priority Date: 10/20/2000
• Status: Expired due to Fees

• Current Assignee: Millennium Pharmaceuticals Incorporated (Takeda Pharmaceutical Company Limited)
• Original Assignee: Millennium Pharmaceuticals Incorporated (Takeda Pharmaceutical Company Limited)
• Inventors: Meyers, Rachel, Glucksmann, Maria A.
• Primary Examiner(s): Saidha, Tekchand
• Application Number:
  US10/080,960
• Publication Number:
  US 20020197695A1
• Time in Patent Office: 1,530 Days
• Field of Search: 435/193, 435/69.1, 435/252.3, 435/320.1, 536/23.1, 536/23.2, 530/350
• US Class Current: 435/193
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C12N 9/10 Transferases (2.) ribonucleases C12N9/22
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)

Abstract

The invention provides isolated nucleic acids molecules, designated 80090, 52874, 52880, 63497, or 33425 nucleic acid molecules, which encode novel fucosyltransferase, seven transmembrane receptor, or RhoGAP family members. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 80090, 52874, 52880, 63497, or 33425 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which an 80090, 52874, 52880, 63497, or 33425 gene has been introduced or disrupted. The invention still further provides isolated 80090, 52874, 52880, 63497, or 33425 proteins, fusion proteins, antigenic peptides and anti-80090, 52874, 52880, 63497, or 33425 antibodies. Diagnostic methods utilizing compositions of the invention are also provided.

Claims

1. An isolated nucleic acid molecule selected from the group consisting of:

a) a nucleic acid molecule comprising the nucleic acid sequence of SEQ ID NO;

1 or SEQ ID NO;

3, or a full complement thereof; and

b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO;

2, or a full complement of the nucleic acid.

3. A vector comprising the nucleic acid molecule of claim 1.

5. The nucleic acid molecule of claim 1, further comprising a nucleic acid sequences encoding a heterologous polypeptide.

7. An isolated host cell which contains the nucleic acid molecule of claim 3.

8. The host cell of claim 7 which is a mammalian host cell.

11. A method for producing a polypeptide, comprising culturing the host cell of claim 7 under conditions in which the polypeptide encoded by the nucleic acid molecule is expressed.

2. An isolated nucleic acid molecule selected from the group consisting of:

a) a nucleic acid molecule consisting of the nucleic acid sequence of SEQ ID NO;

1 or SEQ ID NO;

3, or a full complement thereof; and

b) a nucleic acid molecule which encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO;

2, or a full complement of the nucleic acid.

4. A vector comprising the nucleic acid molecule of claim 2.

6. The nucleic acid molecule of claim 2, further comprising a nucleic acid sequences encoding a heterologous polypeptide.

9. An isolated host cell which contains the nucleic acid molecule of claim 4.

10. The host cell of claim 9 which is a mammalian host cell.

12. A method for producing a polypeptide, comprising culturing the host cell of claim 9 under conditions in which the polypeptide encoded by the nucleic acid molecule is expressed.