Fiber array and methods for using and making same

• US 6,982,149 B2
• Filed: 06/23/2003
• Issued: 01/03/2006
• Est. Priority Date: 01/08/1999
• Status: Expired due to Term

• Current Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Vann, Charles S., Blasband, Andrew J., Geiser, Tim
• Primary Examiner(s): LU, FRANK WEI MIN
• Application Number:
  US10/602,900
• Publication Number:
  US 20030232381A1
• Time in Patent Office: 925 Days
• Field of Search: 435/6, 435/7.1, 435/91.1, 435/183, 435/283.1, 435/287.1, 435/287.2, 435/288.4, 435/288.7, 436/94, 536/23.1, 536/24.3, 536/24.33, 536/25.3, 530/300, 530/350, 530/387.1
• US Class Current: 435/6.19
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00326 Movement by rotation
• CPC Class Codes: B01J 2219/0043 for direct application of reagents, e.g. through openings in a shutter
• CPC Class Codes: B01J 2219/00495 Means for heating or cooling the reaction vessels
• CPC Class Codes: B01J 2219/00515 in the shape of strings
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00628 Ionic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00635 by reactive plasma treatment
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00657 One-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01L 3/5023 : with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6816 : characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2565/607 : being a sensor, e.g. electrode
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 50/14 : Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 2021/6484 : Optical fibres
• CPC Class Codes: G01N 2021/7786 : Fluorescence
• CPC Class Codes: G01N 21/6428 : Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 : Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6452 : Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/648 : using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/7703 : using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]

Abstract

An apparatus and method are provided for contacting at least two chemical species, comprising a support plate having a channel for receiving a mobile chemical species and a fiber, having a second chemical species immobilized thereon, disposed on the support plate. At least a portion of the fiber is exposed to the channel such that the mobile chemical species is capable of contacting the second chemical species. An apparatus and method for reading the fiber array, an apparatus and method for making the fiber array, and methods of using the fiber array of the present invention are also provided.

Claims

1. A method for contacting at least two chemical species, comprising:

immobilizing a first chemical species on a fiber;

placing said fiber on a support across a width of a channel formed in said support; and

disposing a mobile second chemical species into said channel such that said second chemical species contacts said immobilized chemical species on said fiber.

2. The method of claim 1, further comprising providing a plurality of fibers and channels, wherein said immobilizing step comprises immobilizing a different polynucleotide on each of said fibers.

3. The method of claim 1 further comprising the step of moving said second chemical species along said channel.

4. The method of claim 3 wherein said moving step includes the step of applying electro-osmotic force to said channel.

5. A method for analyzing the contact between at least two chemical species, comprising:

immobilizing an immobilized chemical species on at least a first one of a plurality of optical fibers;

placing said plurality of fibers on a support having a plurality of channels, where each fiber of said plurality of fibers is oriented across a width of one or more of said channels;

disposing a mobile chemical species into at least a first one of said plurality of channels such that said mobile chemical species contacts at least said first one of a plurality of optical fibers;

directing light to an end of said at least a first one of a plurality of optical fibers; and

viewing the light emitted from said at least a first one of a plurality of optical fibers.

6. The method of claim 5 wherein said directing step is accomplished by directing said light through a focusing lens.

7. A method for detecting the binding of two chemical species, comprising the steps of:

contacting a first chemical species mobile within a channel with an second chemical species immobilized on a fiber oriented across a width of the channel;

directing light at an end of said fiber; and

detecting light emitted from binding occurring between said first chemical species and said second chemical species.

8. The method of claim 7, wherein said detecting step comprises the steps of:

collecting said light emitted from the binding to produce collected light; and

converting said collected light into an electrical signal that is proportional to said collected light.

9. The method of claim 8, wherein said detecting step comprises the step of detecting light emitted from said binding at a plurality of predetermined locations along said fiber.

10. The method of claim 8, further comprising, after said contacting, gradually changing the temperature of the fiber over a predetermined temperature range such that the temperature of said fiber passes through an optimum temperature for binding of said first and second chemical species.

11. A method according to claim 8, further comprising verifying the chemical composition of said immobilized chemical species or said one or more mobile chemical species.