Altered antibodies
First Claim
1. An antibody comprising a human kappa light chain variable domain, wherein said light chain variable domain comprises a set of tour human Kabat framework regions (FRs), wherein each of said FRs in said set of four FRs comprises residues identical to the most common residue in each position in a Kabat alignment of a human kappa sub-group 1, and wherein said antibody binds an antigen.
1 Assignment
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Accused Products
Abstract
An altered antibody is produced by replacing the complementarity determining regions (CDRs) of a variable region of an immunoglobulin (Ig) with the CDRs from an Ig of different specificity, using recombinant DNA techniques. The gene coding sequence for producing the altered antibody may be produced by site-directed mutagenesis using long oligonucleotides or using gene synthesis.
422 Citations
24 Claims
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1. An antibody comprising a human kappa light chain variable domain, wherein said light chain variable domain comprises a set of tour human Kabat framework regions (FRs), wherein each of said FRs in said set of four FRs comprises residues identical to the most common residue in each position in a Kabat alignment of a human kappa sub-group 1, and wherein said antibody binds an antigen.
- 2. An antibody comprising a human kappa light chain variable domain, said light chain variable domain comprising a set of four human Kabat framework regions (FRs), wherein said antibody incorporates FRs comprising residues identical to the most common residue in each position in a Kabat alignment of a human kappa sub-group 1, and wherein said antibody has three Kabat complementarity determining regions (CDRs) comprising sequences of amino acid residues, wherein said sequences are not human, and wherein said antibody binds the same antigen as a non-human antibody having a kappa light chain comprising said sequences.
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3. An antibody comprising a human kappa light chain variable domain, said light chain variable domain comprising a set of four human Kabat framework regions (FRs), wherein said antibody incorporates FRs comprising residues identical to the most common residue in each position in a Kabat alignment of a human kappa sub-group 1, wherein said antibody has three Kabat complementarity determining regions (CDRs) comprising amino acid residues from a non-human antibody, and wherein said antibody binds the same antigen as the non-human antibody.
- 22. An antibody comprising a human kappa light chain variable domain having a set of four framework regions (FRs) of a human antibody and three complementarity determining regions (CDRs) comprising residues from a non-human antibody, wherein said antibody binds the same antigen as the non-human antibody.
Specification