Optically similar reference samples and related methods for multivariate calibration models used in optical spectroscopy
First Claim
1. A reference sample for maintaining prediction performance of an optical system used to measure an analyte or attribute in a representative measurement sample, wherein the representative measurement sample comprises a bodily tissue, bodily fluid or other biological sample containing the analyte or attribute and having a dominant absorbing species therein when in vivo, the reference sample comprising the dominant absorbing species contained and disposed such that an optical signal interrogating the reference sample is subjected to diffuse reflection, where the reference sample and the measurement sample absorb light at each of selected wavelengths in a manner to produce similarly shaped spectra over the wavelengths measured, wherein the reference sample has the spectral characteristics of an in vivo sample.
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Abstract
Systems and methods for establishing and/or maintaining the prediction capability over time of a multivariate calibration model designed for quantitative optical spectroscopic measurement of attributes or analytes in bodily tissues, bodily fluids or other biological samples, which are particularly useful when the spectral absorbance of the attribute or analyte is small relative to the background. The present invention provides an optically similar reference sample to capture the characteristics of instrument and environmental variation and to reduce the effect of such variation on the measurement capability of the model. The optically similar reference is preferably stable over time and is designed such that its optical properties are sufficiently matched to the sample of interest that instrument and environmental variations are captured in the same manner in both the test sample of interest and the optically similar reference sample. The optically similar reference sample may include one or more physical components which are spectroscopically measured in a manner which closely mimics the spectroscopic measurement of the test sample of interest. Spectral similarity may also be achieved by using alternative components with spectral characteristics similar to the components contained in the test sample of interest.
570 Citations
86 Claims
- 1. A reference sample for maintaining prediction performance of an optical system used to measure an analyte or attribute in a representative measurement sample, wherein the representative measurement sample comprises a bodily tissue, bodily fluid or other biological sample containing the analyte or attribute and having a dominant absorbing species therein when in vivo, the reference sample comprising the dominant absorbing species contained and disposed such that an optical signal interrogating the reference sample is subjected to diffuse reflection, where the reference sample and the measurement sample absorb light at each of selected wavelengths in a manner to produce similarly shaped spectra over the wavelengths measured, wherein the reference sample has the spectral characteristics of an in vivo sample.
- 34. A reference sample for maintaining prediction performance of an optical system used to measure an analyte or attribute in a representative measurement sample, the reference sample including a dominant absorbing species housed in a structure defining a plurality of dispersive optical interfaces, the dominant absorbing species corresponding to a dominant absorbing material present in the representative measurement sample when in vivo, wherein the representative measurement sample comprise a bodily tissue, bodily fluid or other biological sample containing the analyte or attribute, where the reference sample simulates the optical interaction between the measurement sample and the optical system.
- 41. A reference sample for maintaining prediction performance of an optical system to measure an analyte or attribute in a representative measurement sample, wherein the representative measurement sample comprises a bodily tissue, bodily fluid or other biological sample containing the analyte or attribute as well as a dominant absorbing species present when the representative measurement sample is in vivo, where the reference sample has the same primary optical absorber as the measurement sample, wherein the reference sample has the spectral characteristics of an in vivo sample, the dominant absorbing species being the primary optical absorber, the dominant absorbing species being contained in a housing allowing optical interrogation thereof.
- 47. A reference sample for maintaining prediction performance of an optical system used to measure an analyte or attribute in a representative measurement sample, wherein the representative measurement sample comprises a bodily tissue, bodily fluid or other biological sample containing the analyte or attribute, with the reference sample including a structure having one or more optically interactive surfaces and producing a non-stepwise reference spectrum that is optically similar to the representative measurement sample, both the reference sample and the representative measurement sample having a primary optical absorber corresponding to a dominant in vivo optical absorber for the representative measurement sample.
- 78. A reference sample for maintaining prediction performance or an optical system used to measure an analyte or attribute in a test sample of interest, wherein the test sample comprises a bodily tissue, bodily fluid or other biological sample containing the analyte or attribute, with the reference sample producing a reference sample spectrum that is similar to the test sample spectrum, the reference sample including a transmissive optical interface and an optical sampling compartment, the optical sampling compartment containing water and a diffusely reflective or scattering media and including structure such that an optical signal passing through the transmissive optical interface encounters reflective surfaces at various path lengths from the optical interface.
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83. A reference device for use with an optical system to maintain prediction performance of the optical system for a constituent of a representative measurement sample, the reference device comprising:
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a reference material having a first optical similarity to the representative measurement sample; and
a structure for containing the reference material in a geometric configuration adapted to give the reference device a second optical similarity to the representative measurement sample;
wherein the second optical similarity is greater than the first optical similarity. - View Dependent Claims (84, 85, 86)
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Specification