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Multilayered microfluidic DNA analysis system and method

  • US 6,984,516 B2
  • Filed: 01/10/2003
  • Issued: 01/10/2006
  • Est. Priority Date: 10/09/1998
  • Status: Expired due to Fees
First Claim
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1. A multilayered microfluidic DNA amplification device comprising:

  • a monolithic structure formed from a plurality of green-sheet layers sintered together, said green-sheet layers including particles selected from the group consisting of ceramic particles, glass particles, and glass-ceramic, particles;

    said monolithic structure having a fluid passageway defined therein, said fluid passageway including an inlet port for receiving fluid and a DNA amplification chamber for amplifying DNA in said fluid;

    at least one fluid control system contiguous to the fluid passageway for providing electroosmotic pumping to transport the fluid therealong; and

    said monolithic structure having defined therein a means for heating said DNA amplification chamber and a means for cooling said DNA amplification chamber, said heating and cooling means subjecting the DNA to one or more cycles of a temperature regimen.

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