Multilayered microfluidic DNA analysis system and method
First Claim
1. A multilayered microfluidic DNA amplification device comprising:
- a monolithic structure formed from a plurality of green-sheet layers sintered together, said green-sheet layers including particles selected from the group consisting of ceramic particles, glass particles, and glass-ceramic, particles;
said monolithic structure having a fluid passageway defined therein, said fluid passageway including an inlet port for receiving fluid and a DNA amplification chamber for amplifying DNA in said fluid;
at least one fluid control system contiguous to the fluid passageway for providing electroosmotic pumping to transport the fluid therealong; and
said monolithic structure having defined therein a means for heating said DNA amplification chamber and a means for cooling said DNA amplification chamber, said heating and cooling means subjecting the DNA to one or more cycles of a temperature regimen.
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Accused Products
Abstract
A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together. The substantially monolithic structure has defined therein a means for heating the DNA amplification chamber and a means for cooling the DNA amplification chamber. The means for heating and means for cooling operate to cycle the temperature of the DNA amplification chamber as required for performing a DNA amplification process, such as PCR.
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Citations
29 Claims
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1. A multilayered microfluidic DNA amplification device comprising:
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a monolithic structure formed from a plurality of green-sheet layers sintered together, said green-sheet layers including particles selected from the group consisting of ceramic particles, glass particles, and glass-ceramic, particles; said monolithic structure having a fluid passageway defined therein, said fluid passageway including an inlet port for receiving fluid and a DNA amplification chamber for amplifying DNA in said fluid; at least one fluid control system contiguous to the fluid passageway for providing electroosmotic pumping to transport the fluid therealong; and said monolithic structure having defined therein a means for heating said DNA amplification chamber and a means for cooling said DNA amplification chamber, said heating and cooling means subjecting the DNA to one or more cycles of a temperature regimen. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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29. A multilayered microfluidic DNA amplification device comprising:
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a monolithic structure formed from a plurality of green-sheet layers sintered together, said green-sheet layers including particles selected from the group consisting of ceramic particles, glass particles, and glass-ceramic particles; said monolithic structure having a fluid passageway defined therein, said fluid passageway including an inlet port for receiving the fluid, a cell lysis chamber for receiving the fluid from the inlet port, a DNA separation chamber, a buffer injection port and a first waste outlet port coupled to the DNA separation chamber, a DNA amplification chamber, a regent injection port and a second waste outlet port coupled to the DNA amplififcation chamber, and a DNA detection chamber; one or more electric field generation elements positioned in the cell lysis chamber; a first fluid control system contiguous to the fluid passageway for providing electroosmotic pumping to transport the fluid from the cell lysis chamber to the DNA separation chamber; a magnetic field element contiguous to the DNA separation chamber; a second fluid control system contiguous to the fluid passageway for providing electroosmotic pumping to transport the fluid from the DNA seperation chamber to the DNA amplififcation chamber; a heating and cooling element contiguous to the DNA amplification chamber for subjecting the DNA to a plurality of cycles of a temperature regimen; a third fluid control system contiguous to the fluid passageway for providing electroosmotic pumping to transport the fluid from the DNA amplification chamber to the DNA detection chamber; and a transparent material positioned adjacent the DNA detection chamber allowing optical access thereto.
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Specification