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Method of determining the concentration of an analyte in a physiological sample

  • US 6,989,243 B2
  • Filed: 05/24/2002
  • Issued: 01/24/2006
  • Est. Priority Date: 02/02/2000
  • Status: Expired due to Term
First Claim
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1. A method of determining a analyte concentration in a physiological sample, said method comprising:

  • (a) applying said physiological sample to a test strip comprising;

    (i) a porous matrix;

    (ii) at least one member of an analyte oxidation signal producing system; and

    (iii) at least one hemolyzing agent, wherein said hemolyzing agent is present in an amount sufficient to produce hemolysis which is equivalent to a hemolysate concentration in a physiological sample applied to said porous matrix in an amount ranging from about 5 to 20% by volume;

    (b) detecting a signal produced by said signal producing system; and

    (c) relating said detected signal to the analyte concentration in said physiological sample.

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