Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
First Claim
1. A method for detecting a secondary component in a sample comprising the steps of:
- a) contacting a sample containing or suspected to contain the secondary component to be detected with a primary component under conditions to form a complementary binding pair and wherein said primary component is labelled with a luminescent dye selected from the group consisting of cyanine, merocyanine, styryl and oxonal dyes containing at least one sulphonate or sulphonic acid attached to an aromatic nucleus which dye is reactive with said primary component, wherein said dye has a molar extinction coefficient of at least 50,000 liters per mole centimeter, quantum yield of at least 5 percent and absorbs and emits maximally in the 400 to 900 nm range; and
b) detecting the binding of said secondary component to said primary component by;
i) illuminating the sample with light; and
ii) detecting the emitted fluorescence from said sample.
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Abstract
The present invention pertains to luminescent dyes and methods for covalently attaching the dyes to a component or mixture of components so that the components may be detected and/or quantified by luminescence detection methods. The dyes are cyanine and cyanine-type dyes that contain or are derivatized to contain a reactive group. The reactive group is covalently reactive with amine, hydroxy and/or sulfhydryl groups on the component so that the dye can be covalently bound to the component. In addition, the dyes are preferably soluble in aqueous or other medium in which the component is contained. The components to be labeled can be either biological materials, such as antibodies, antigens, peptides, nucleotides, hormones, drugs, or non-biological materials, such as polymers, glass, or other surfaces. Any luminescent or light absorbing detecting step can be employed in the method of the invention.
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Citations
4 Claims
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1. A method for detecting a secondary component in a sample comprising the steps of:
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a) contacting a sample containing or suspected to contain the secondary component to be detected with a primary component under conditions to form a complementary binding pair and wherein said primary component is labelled with a luminescent dye selected from the group consisting of cyanine, merocyanine, styryl and oxonal dyes containing at least one sulphonate or sulphonic acid attached to an aromatic nucleus which dye is reactive with said primary component, wherein said dye has a molar extinction coefficient of at least 50,000 liters per mole centimeter, quantum yield of at least 5 percent and absorbs and emits maximally in the 400 to 900 nm range; and b) detecting the binding of said secondary component to said primary component by; i) illuminating the sample with light; and ii) detecting the emitted fluorescence from said sample. - View Dependent Claims (2)
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3. A method for labeling and detecting a component of an aqueous liquid comprising:
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a) adding to said liquid containing said component a luminescent dye selected from the group consisting of cyanine, merocyanine, styryl and oxonol dyes containing at least one sulphonate or sulphonic acid attached to an aromatic nucleus which dye is reactive with said component; b) reacting said dye with said component so that said dye labels said component, wherein dye has a molar extinction coefficient of at least 50,000 liters per mole centimeter, quantum yield of at least 5 percent and absorbs and emits maximally in the 400 to 900 nm range; and c) detecting said component by; i) illuminating said labeled component with light; and ii) detecting light that is fluoresced by said labeled component. - View Dependent Claims (4)
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Specification