Optical array device and methods of use thereof for screening, analysis and manipulation of particles

• US 6,991,939 B2
• Filed: 07/19/2002
• Issued: 01/31/2006
• Est. Priority Date: 07/19/2001
• Status: Expired due to Fees

• Current Assignee: Tufts University
• Original Assignee: Tufts University
• Inventors: Weissman, Irving L., Walt, David R., Biran, Israel, Tam, Jenny
• Primary Examiner(s): Snay, Jeffrey R.
• Application Number:
  US10/199,341
• Publication Number:
  US 20030032204A1
• Time in Patent Office: 1,292 Days
• Field of Search: 250/251, 250/458.1, 250/459.1, 436/518, 436/536, 436/537, 436/164, 436/165, 436/172, 436/174, 436/180, 422/82.05, 422/82.08, 422/99, 422/100
• US Class Current: 436/172
• CPC Class Codes: G21K 1/006 Manipulation of neutral particles by using radiation pressure, e.g. optical levitation production or acceleration of neutral particles H05H3/00
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

Methods and devices are provided for the trapping, including optical trapping; analysis; and selective manipulation of particles on an optical array. A multi-channel device parcels a light source into many points of light transmitted through an optical array of fibers or conduits, preferably where the individual points of light are individually controllable through a light controlling device. Optical properties of the particles may be determined by interrogation with light focused through the optical array. The particles may be manipulated by immobilizing or releasing specific particles, separating types of particles, etc.

Claims

1. A device for parallel trapping of multiple dielectric particles, comprising:

an optical array comprising a plurality of strands disposed coaxially along their lengths to form a single, discrete construction, wherein said array parcels a beam of light into individual beams of light, wherein the distal terminus of each strand is light focusing and wherein each strand is connectable to a detector.

2. The device according to claim 1, wherein said optical array comprises a plurality of optical fibers.

3. The device according to claim 1, wherein said optical array comprises a plurality of optical conduits.

4. The device according to claim 2, wherein said optical fibers are self-focusing.

5. The device according to claim 2, wherein said optical fibers comprise a light focusing element.

6. The device according to claim 5, wherein said light focusing element comprises a lens selected from the group consisting of polymeric coating, ball lens, cylindrical fiber lens, aspherical fiber lens, and photothermally generated lens array.

7. The device according to claim 1, further comprising a light controlling device.

8. The device according to claim 7, wherein said light controlling device comprises a digital micromirror device comprising an array of electrostatically actuated tiltable micromirrors, wherein a beam of light is reflected by said micromirrors individually into said strands, and wherein said micromirrors can further be tilted to reflect said beam of light away from said optical array.

9. The device according to claim 1, further comprising:

a fluidics system.

10. The device according to claim 9, wherein said fluidics system comprises a fluidic channel for recirculation of a sample comprising said multiple dielectric particles.

11. The device according to claim 10, wherein said fluidics system further comprises one or both of a supply vessel and a collection vessel.

12. The device according to claim 9, further comprising a pumping means.

13. A device according to claim 9, wherein said device is enclosed within a sealed container.

14. The device according to claim 1, further comprising a light detector operably coupled to said proximal or distal terminus of each said strand.

15. The device according to claim 14, wherein said light detector is selected from the group consisting of photomultipliers, diode arrays and charge coupled devices.

16. The device according to claim 15, further comprising a light source operably coupled to said multi-channel device.

17. The device according to claim 16, further comprising a data processing element operably connected to said light detector and to said multi-channel device.

18. A method for analysis of the optical properties of a population of dielectric particles, the method comprising:

dispersing a population of said dielectric particles on a device according to claim 1;

optically trapping said particles;

illuminating said particles;

detecting emitted light from individual particles;

wherein said emitted light is indicative of the optical properties of said individual particles.

19. The method according to claim 18, wherein said light controlling device comprises a digital micromirror device comprising an array of electrostatically actuated tiltable micromirrors, wherein a beam of light is reflected by said micromirrors individually into said strands, and wherein said micromirrors can further be tilted to reflect said beam of light away from said optical array.

20. The method according to claim 18, wherein said population of dielectric particles comprises cells or cellular particles.

21. The method according to claim 20, wherein said cell population is a complex population comprising two or more phenotypically distinct cells.

22. The method according to claim 18, wherein said dielectric particles comprise agents for screening.

23. The method according to claim 18, wherein said dielectric particles comprise one of a pair of binding members.

24. The method according to claim 23, wherein said dielectric particles comprise a receptor.

25. The method according to claim 24, further comprising the step of contacting said dielectric particles with mobile phase ligand and detecting the presence of interaction between a receptor and ligand.

26. The method according to claim 18, wherein said dielectric particles comprise a ligand.

27. The method according to claim 26, further comprising the step of contacting said dielectric particles with mobile phase receptor and detecting the presence of interaction between a receptor and ligand.

28. The method of claim 18, wherein said optical properties comprise the presence of fluorescent labels.

29. The method of claim 28, wherein said fluorescent labels are bound to antibodies specific for cell surface molecules.

30. The method according to claim 18, further comprising the step of selectively releasing a subset of said particle population, wherein said selectivity is determined by the presence of optical properties on said particles, and wherein said releasing step comprises turning off a trapping light beam.

31. The method of claim 30, further comprising the step of:

collecting said subset after said releasing step.