Composite arrays utilizing microspheres
First Claim
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1. A method of making a composite array composition comprising:
- a) providing a substrate with a surface comprising a plurality of assay locations, each assay location comprising an array location, said array location comprising a plurality of discrete sites; and
b) providing a population of microspheres comprising at least a first and a second subpopulation, wherein said first subpopulation comprises a first bioactive agent and wherein said second subpopulation comprises a second bioactive agent;
wherein said microspheres are randomly distributed in said discrete sites in said array location; and
c) contacting said randomly distributed microspheres with a plurality of different decoder binding ligands, whereby the location of said bioactive agents is determined.
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Abstract
The invention relates to compositions and methods for decoding microsphere array sensors.
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Citations
21 Claims
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1. A method of making a composite array composition comprising:
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a) providing a substrate with a surface comprising a plurality of assay locations, each assay location comprising an array location, said array location comprising a plurality of discrete sites; and
b) providing a population of microspheres comprising at least a first and a second subpopulation, wherein said first subpopulation comprises a first bioactive agent and wherein said second subpopulation comprises a second bioactive agent;
wherein said microspheres are randomly distributed in said discrete sites in said array location; and
c) contacting said randomly distributed microspheres with a plurality of different decoder binding ligands, whereby the location of said bioactive agents is determined. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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2. A method of making a composite array composition comprising:
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a) randomly distributing a population of microspheres on a substrate with a surface comprising a penalty of assay locations, each assay location comprising an array location, said array location comprising a plurality of discrete sites, wherein said population comprises a first subpopulation of microspheres comprising a first bioactive agent and a second subpopulation of microspheres comprising a second bioactive agent; and
a) following said distributing, contacting said microspheres with a plurality of different decoder binding ligands, whereby the location of said bioactive agents is identified.
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3. A method of making a composite array composition comprising:
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b) providing a composite array comprising;
i) a substrate with a surface comprising a plurality of assay location, each assay location comprising an array location, said array location comprising a plurality of discrete sites; and
ii) a population of microspheres comprising at least a first and a second subpopulation, wherein said first subpopulation comprises a first bioactive agent and wherein said second subpopulation comprises a second bioactive agent, wherein said microspheres are randomly distributed in said discrete sites in said array location; and
b) following said providing contacting said microspheres with a plurality of different decoder binding ligands, whereby the location of said bioactive agents is identified.
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Specification