Optical sensor and method for measuring concentration of a chemical constituent using its intrinsic optical absorbance

• US 7,002,670 B2
• Filed: 06/12/2002
• Issued: 02/21/2006
• Est. Priority Date: 06/12/2002
• Status: Expired due to Fees

• Current Assignee: Baxter Healthcare SA (Baxter International Inc.), Baxter International Inc.
• Original Assignee: Baxter Healthcare SA (Baxter International Inc.), Baxter International Inc.
• Inventors: Wariar, Ramesh, Pan, Li
• Primary Examiner(s): Lauchman, Layla G.
• Application Number:
  US10/167,796
• Publication Number:
  US 20030231294A1
• Time in Patent Office: 1,350 Days
• Field of Search: 356 39- 41, 356/432, 356/433, 356/435, 356/436, 356/440, 356/441, 356/442, 250/573, 250/574, 435/4, 435/5, 435/7, 435/26, 435/808
• US Class Current: 356/39
• CPC Class Codes: C12Q 1/34 involving hydrolase
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/75 Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated systems in which material is burnt in a flame or plasma G01N21/72, G01N21/73
• CPC Class Codes: G01N 21/85 Investigating moving fluids or granular solids

Abstract

An improved optical sensor and method for measuring concentration of a chemical constituent where measurement interference from other chemical compounds is present in the solution is provided. More specifically, the invention relates to a system for measuring the amount of creatinine in effluent dialysate during, before, or following a kidney dialysis procedure and a method for using the same. Alternatively, the method may be used with blood and other body fluids or solutions that contact the patient. The system may use an enzyme or other chemical process to specifically remove or convert an analyte with an intrinsic optical absorbance. By measuring the absorbance before and after the chemical process the analyte can be measured with high accuracy and specificity. The method may be extended to multiple analyte measurements using cascaded chemical processes.

Claims

1. A system for measuring an analyte in a biological fluid using an intrinsic optical absorbance of the analyte, the system comprising:

a fluid sample containing an analyte;

a light source forming an optical beam that is directed on a route through the fluid sample;

a reactor that selectively removes the optical absorbance of the analyte wherein the analyte has a first optical absorbance before the removal and further having a second optical absorbance after the removal; and

a light detector receiving the optical beam directed through the fluid sample to produce a first signal indicative of the first optical absorbance and receiving the optical beam directed through the fluid sample after the removal to produce a second signal indicative of the second optical absorbance.

2. The system of claim 1 further comprising:

a processor comparing the first optical absorbance to the second optical absorbance.

3. The system of claim 1 further comprising:

a cuvette through which the fluid sample is directed.

4. The system of claim 1 further comprising:

a cuvette through which the fluid sample is directed after the process.

5. The system of claim 1 wherein the reactor includes an enzyme.

6. The system of claim 1 further comprising:

a beamsplitter in the route of the optical beam separating the optical beam into a first beam and a second beam.

7. The system of claim 1 further comprising:

a mirror in the route of the optical beam, the mirror reflecting the optical beam to the light detector.

8. The system of claim 1 wherein multiple analytes from the fluid sample are measured.

9. The system of claim 1 wherein an optical cuvette is associated with a valve to measure the fluid sample before and after the removal.

10. The system of claim 5 wherein the enzyme is immobilized.

21. The system of claim 5 wherein the enzyme is creatinine deiminase.

11. A method for measuring creatinine, the method comprising the steps of:

pumping a substance through an optical beam;

detecting a first optical absorbance of the substance;

combining the substance with creatinine deiminase;

detecting a second optical absorbance of the substance combined with creatinine deiminase; and

comparing the first optical absorbance to the second optical absorbance.

12. The method of claim 11 further comprising the step of:

pumping the substance through a cuvette.

13. The method of claim 11 further comprising the step of:

directing the optical beam with an optical waveguide.

14. The method of claim 11 further comprising the step of:

reflecting the optical beam with a mirror.

15. The method of claim 11 further comprising the step of:

directing the optical beam with a beam splitter that splits the optical beam into a first beam and a second beam.

16. The method of claim 11 further comprising the step of:

obtaining the substance from a body fluid, body secretion or from clinical therapies for example renal-replacement.

17. The method of claim 11 further comprising the step of:

obtaining the substance from a patient.

18. The method of claim 11 further comprising the step of:

intermittently measuring the first optical absorbance and the second optical absorbance.

19. The method of claim 11 further comprising the step of:

continuously measuring the first optical absorbance and the second optical absorbance.

20. The method of claim 11 wherein creatinine deiminase is combined with creatinine in the substance to produce at least one of N-methylhydantoin and NH₃.