Glucose sensor

• US 7,005,048 B1
• Filed: 10/02/2000
• Issued: 02/28/2006
• Est. Priority Date: 10/05/1999
• Status: Expired due to Term

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Miyazaki, Shoji, Nakayama, Junko, Yoshioka, Toshihiko, Nankai, Shiro, Yugawa, Keiko, Watanabe, Motokazu, Baba, Hideyuki
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/807,692
• Time in Patent Office: 1,975 Days
• Field of Search: 204/403, 204/403.14, 204/403.09, 204/403.1, 204/403.04, 204/403.11
• US Class Current: 204/403.14
• CPC Class Codes: C12Q 1/006 for glucose

Abstract

The present invention provides a high-performance glucose sensor having excellent storage stability and an improved response characteristic. This sensor comprises: an electrically insulating base plate; an electrode system including at least a working electrode and a counter electrode formed on the base plate; and a reaction layer containing at least pyrrolo-quinoline quinone dependent glucose dehydrogenase, formed in contact with or in the vicinity of the electrode system, and the reaction layer contains at least one kind of additive selected from the group consisting of gluconic acid and salts thereof.

Claims

1. A glucose sensor comprising:

an electrically insulating base plate;

an electrode system including at least a working electrode and a counter electrode formed on said base plate; and

a reaction layer formed in contact with or in the vicinity of said electrode system, wherein said reaction layer contains an admixture of at least pyrrolo-quinoline quinone dependent glucose dehydrogenase and potassium gluconate and wherein the glucose sensor is in a sealed container.

2. The glucose sensor as set forth in claim 1, wherein said reaction layer further contains at least one additive selected from the group consisting of phthalic acid, salts of phthalic acid, maleic acid, salts of maleic acid, succinic acid and salts of succinic acid.

3. The glucose sensor as set forth in claim 2, wherein said reaction layer further contains calcium ions.

4. The glucose sensor as set forth in claim 2, wherein said reaction layer further contains an electron mediator.

5. The glucose sensor as set forth in claim 1, wherein said reaction layer further contains calcium ions.

6. The glucose sensor as set forth in claim 5, wherein said reaction layer further contains an electron mediator.

7. The glucose sensor as set forth in claim 1, wherein said reaction layer further contains an electron mediator.

8. The glucose sensor as set forth in claim 1, wherein said reaction layer further contains an electron mediator.

9. The glucose sensor as set forth in claim 1 wherein the amount of potassium gluconate is within the range of 1.5 to 150 μ

g and the amount of glucose dehydrogenase is 0.2 to 20 U.

10. A glucose sensor comprising:

an electrically insulating base plate;

an electrode system including at least a working electrode and a counter electrode formed on said base plate; and

a reaction layer formed in contact with or in the vicinity of said electrode system, wherein said reaction layer contains an admixture of at least pyrrolo-quinoline quinone dependent glucose dehydrogenase and potassium gluconate, wherein the response of the sensor immediately fabricated is substantially the same as compared to the sensor after being stored for one week at 40°

C.

11. The glucose sensor as set forth in claim 10 in a sealed container.

12. The glucose sensor as set forth in claim 10 wherein the amount of potassium gluconate is within the range of 1.5 to 150 μ

g and the amount of glucose dehydrogenase is 0.2 to 20 U.

13. A glucose sensor comprising:

an electrically insulating base plate;

an electrode system including at least a working electrode and a counter electrode formed on said base plate; and

a reaction layer containing at least pyrrolo-quinoline quinone dependent glucose dehydrogenase, formed in contact with or in the vicinity of said electrode system, wherein said reaction layer contains potassium gluconate and one additive selected from the group consisting of phthalic acid, salts of phthalic acid, maleic acid, salts of maleic acid, succinic acid and salts of succinic acid.