Lipid particles having asymmetric lipid coating and method of preparing same
First Claim
1. A method of preparing lipid particles having an external lipid coating, comprising:
- preparing lipid particles comprised of (i) a lipid composition containing a charged lipid and (ii) a therapeutic agent, said particles each having an outer lipid coating having an external lipid leaflet and an internal lipid structure; and
incubating said particles in a medium and under conditions effective to remove said charged lipid from the external lipid leaflet into said medium.
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Accused Products
Abstract
A method of preparing lipid particles having an asymmetric lipid coating is described. The lipid composition of the outer lipid coating of the particles varies from the inner to outer surfaces. The asymmetric lipid particles are formed by preparing a lipid composition containing a charged lipid and a therapeutic agent, where the particles each have an outer lipid coating with an external lipid leaflet and an internal lipid structure. The particles are then incubated under conditions effective to remove the charged lipid from the external lipid leaflet, thus rendering the lipid coating asymmetric. The particles have the ability to their regain surface charge via translocation of the lipids.
34 Citations
18 Claims
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1. A method of preparing lipid particles having an external lipid coating, comprising:
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preparing lipid particles comprised of (i) a lipid composition containing a charged lipid and (ii) a therapeutic agent, said particles each having an outer lipid coating having an external lipid leaflet and an internal lipid structure; and incubating said particles in a medium and under conditions effective to remove said charged lipid from the external lipid leaflet into said medium. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 18)
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13. A method of preparing lipid particles having an asymmetric charged lipid composition in its outer lipid coating prior to in vivo administration, comprising:
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preparing lipid particles comprised of (i) a lipid composition containing a charged lipid and (ii) a therapeutic agent, said particles each having an outer lipid coating having an external lipid leaflet and an internal lipid structure; and incubating said particles in a medium and under conditions effective to remove charged lipids from the external lipid leaflet into said medium. - View Dependent Claims (14, 15, 16, 17)
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Specification