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Nucleic acid sequence analysis

  • US 7,008,766 B1
  • Filed: 07/24/1998
  • Issued: 03/07/2006
  • Est. Priority Date: 07/28/1997
  • Status: Expired due to Term
First Claim
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1. A method for sequencing a polynucleotide, comprising the steps of:

  • (i) reacting a target polynucleotide with a polymerase, wherein said polymerase is immobilised on a solid support, and nucleotides, under conditions sufficient for the polymerase reaction; and

    (ii) detecting the interaction between the polymerase, the target polynucleotide and each specific nucleotide complementary to the target polynucleotide, that is incorporated into a nascent polynucleotide being synthesized as a result of the polymerase reaction, to thereby determine the sequence of the target polynucleotide, the detection being carried out by using surface plasmon resonance to measure a change in, or absorption of, radiation that occurs during the interaction.

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