Nucleic acid sequence analysis
First Claim
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1. A method for sequencing a polynucleotide, comprising the steps of:
- (i) reacting a target polynucleotide with a polymerase, wherein said polymerase is immobilised on a solid support, and nucleotides, under conditions sufficient for the polymerase reaction; and
(ii) detecting the interaction between the polymerase, the target polynucleotide and each specific nucleotide complementary to the target polynucleotide, that is incorporated into a nascent polynucleotide being synthesized as a result of the polymerase reaction, to thereby determine the sequence of the target polynucleotide, the detection being carried out by using surface plasmon resonance to measure a change in, or absorption of, radiation that occurs during the interaction.
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Abstract
The present invention relates to a method for determining the sequence of a polynucleotide, the method comprising the steps of: (i) reacting a target polynucleotide with a polymerase enzyme immobilised on a solid support, and the different nucleotides, under conditions sufficient for the polymerase reaction; and (ii) detecting the incorporation of a specific nucleotide complementary to the target polynucleotide, by measuring radiation.
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Citations
24 Claims
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1. A method for sequencing a polynucleotide, comprising the steps of:
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(i) reacting a target polynucleotide with a polymerase, wherein said polymerase is immobilised on a solid support, and nucleotides, under conditions sufficient for the polymerase reaction; and
(ii) detecting the interaction between the polymerase, the target polynucleotide and each specific nucleotide complementary to the target polynucleotide, that is incorporated into a nascent polynucleotide being synthesized as a result of the polymerase reaction, to thereby determine the sequence of the target polynucleotide, the detection being carried out by using surface plasmon resonance to measure a change in, or absorption of, radiation that occurs during the interaction. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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