Nanoscale molecular arrayer

• US 7,008,769 B2
• Filed: 08/14/2001
• Issued: 03/07/2006
• Est. Priority Date: 08/15/2000
• Status: Expired due to Fees

• Current Assignee: BioForce Nanosciences, Inc.
• Original Assignee: BioForce Nanosciences, Inc.
• Inventors: Henderson, Eric, Mosher, Curtis
• Primary Examiner(s): FORMAN, BETTY J
• Application Number:
  US09/929,865
• Publication Number:
  US 20020076927A1
• Time in Patent Office: 1,666 Days
• Field of Search: 435/4, 435/6, 435/7.1, 435/174, 435/283.1, 435/287.2, 422/68.1, 422/99, 422/100
• US Class Current: 435/6.19
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/00691 using robots
• CPC Class Codes: B01J 2219/00698 Measurement and control of process parameters
• CPC Class Codes: B01J 2219/00704 integrated with the reactor apparatus
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B82B 3/00 Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 40/00 : Manufacture or treatment of nanostructures
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 50/14 : Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
• CPC Class Codes: Y10S 977/924 : using nanostructure as support of dna analysis

Abstract

The present invention is a dedicated apparatus for the formation of array that includes one or more deposition domains comprised of one or more deposition materials. The present invention may include an X, Y controller, an X, Y translation stage, a loading substrate, a deposition substrate, a Z controller, and a deposition probe. A computer controls all of the relative positions of each of the components. Furthermore, the present invention utilizes a humidity control system to create a capillary bridge between the probe and the substrate for transferring the deposition material between the loading substrate, the deposition probe, and the deposition substrate.

Claims

1. An apparatus for creating a molecular array comprising:

a base;

a Z controller coupled to the base, wherein the Z controller is selectively positionable along a Z axis relative to the base;

a deposition probe removably coupled to the Z controller so that the deposition probe is selectively positionable along the Z axis relative to the base by the Z controller;

an X, Y controller coupled to the base, wherein the X, Y controller is selectively positionable in an X-Y plane independently of movement of the Z controller, the X, Y controller further comprising a deposition substrate coupled thereto and wherein the movement of the X, Y controller moves the deposition substrate between a first position and a second position, the second position being located under the deposition probe; and

an X, Y translation stage coupled to the base wherein the X, Y translation stage is selectively positionable in an X-Y plane independently of movement of the X, Y controller, the X, Y translation stage further comprising a loading substrate coupled thereto and wherein the movement of the X, Y translation stage moves the loading substrate between a first position and a second position, the second position being located under the deposition probe.

2. The apparatus of claim 1 further comprising a control computer.

3. The apparatus of claim 2 further comprising a humidity controller coupled to the base wherein the humidity controller controls the humidity around the deposition probe.

4. The apparatus of claim 3 wherein the humidity controller is coupled to the control computer.

5. The apparatus of claim 1 wherein the Z controller has an approximately 200 nanometer spatial resolution along the Z axis.

6. The apparatus of claim 5 wherein the X, Y controller has an approximately 20 nanometer spatial resolution in the X-Y plane.

7. The apparatus of claim 1 wherein the loading substrate further comprises one or more deposition materials deposited thereon.

8. The apparatus of claim 1 further comprising an optical microscope coupled to the base.

9. The apparatus of claim 2 further comprising a force feedback monitor.

10. The apparatus of claim 1 wherein the deposition probe further includes a tip.

11. The apparatus of claim 10 further comprising a humidity controller, the humidity controller selectively controlling the humidity of the air around the tip.

12. The apparatus of claim 2 wherein the control computer further comprises a stepper motor control card.

13. The apparatus of claim 11 wherein the humidity controller further comprises a dry gas source, a humidity source, and a gas flow monitor.

14. An apparatus for creating a molecular array on a deposition substrate comprising:

a base;

a deposition probe removably coupled to the base;

an X, Y translation stage coupled to the base wherein the X, Y translation stage is selectively positionable along the X axis, and the Y axis, the X, Y translation stage further comprising a loading substrate coupled thereto and wherein the movement of the X, Y translation stage moves the loading substrate between a first position and a second position, the second position being located under the deposition probe; and

an X, Y controller coupled to the base wherein the X, Y controller is selectively positionable along the X axis, and the Y axis independently of the X, Y translation stage, the X, Y controller further comprising a deposition substrate coupled thereto and wherein the movement of the X, Y controller moves the deposition substrate between a first position and a second position, the second position being located under the deposition probe.

15. The apparatus of claim 14 further comprising a control computer.

16. The apparatus of claim 15 further comprising a humidity controller coupled to the base wherein the humidity controller controls the humidity around the deposition probe.

17. The apparatus of claim 16 wherein the humidity controller is to the control computer.

18. The apparatus of claim 14 further comprising a Z controller coupled to the base, wherein the Z controller is selectively positionable along a Z axis, and wherein the Z controller has an approximately 200 nanometer spatial resolution along the Z axis.

19. The apparatus of claim 18 wherein at least one of the X, Y controller and the X, Y translation stage have an approximately 20 nanometer spatial resolution along the X and Y axes.

20. The apparatus of claim 14 wherein the loading substrate further comprises one or more deposition materials deposited thereon.

21. The apparatus of claim 14 further comprising an optical microscope coupled to the base.

22. The apparatus of claim 15 further comprising a force feedback monitor.

23. The apparatus of claim 14 wherein the deposition probe further includes a tip.

24. The apparatus of claim 23 further comprising a humidity controller, the humidity controller selectively controlling the humidity of the air around the tip.

25. The apparatus of claim 15 wherein the control computer further comprises a stepper motor control card.

26. The apparatus of claim 25 wherein the humidity controller further comprises a dry gas source, a humidity source, and a gas flow monitor.

27. An apparatus for creating an array on a substrate comprising:

a base;

a deposition probe coupled to the base, the deposition probe further comprising a tip;

an X, Y translation stage coupled to the base and movable in X and Y directions;

a loading substrate coupled to the X, Y translation stage where the loading substrate is selectively movable in the X and Y directions and into a position under the deposition probe;

an X, Y controller coupled to the base and movable in the X and Y directions independently with respect to the X, Y translation stage;

a deposition substrate coupled to the X, Y controller where the deposition substrate is selectively movable by the X, Y controller into a position under the deposition probe; and

a humidity controller, the humidity controller selectively adjusting the humidity around the deposition probe, the X, Y translation stage, and the X, Y controller.

28. An apparatus for creating an array on a substrate, the apparatus comprising:

a base;

a Z controller coupled to the base and movable relative to the base along a Z axis;

a deposition probe removably coupled to the Z controller such that the deposition probe is movable relative to the base along the Z axis;

a loading substrate coupled to the base and movable relative to the deposition probe in an X-Y plane, the loading substrate movable between a first position in the X-Y plane in which the loading substrate is not positioned under the deposition probe and a second position in which the loading substrate is positioned under the deposition probe to allow the deposition probe to pick up material from the loading substrate; and

a deposition substrate coupled to the base and movable relative to the deposition probe in an X-Y plane, the deposition substrate movable independently of movement of the loading substrate between a first position in the X-Y plane in which the deposition substrate is not positioned under the deposition probe and a second position in which the deposition substrate is positioned under the deposition probe to allow the deposition probe to deposit material onto the deposition substrate.