Diagnostics based on tetrazolium compounds
First Claim
1. A reagent for measuring a concentration of an analyte in a hemoglobin-containing biological fluid, comprisinga) a flavin-dependent enzyme that has a flavin bound to it and that has specificity for the analyte, b) a tetrazolium dye precursor, c) an electron transfer agent, and d) a nitrite salt.
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Abstract
A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises a flavin-dependent enzyme that has specificity for the analyte, a flavin cofactor if, and only if, a flavin is not bound to the enzyme, a tetrazolium dye precursor, an electron transfer agent, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring glucose in whole blood.
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Citations
5 Claims
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1. A reagent for measuring a concentration of an analyte in a hemoglobin-containing biological fluid, comprising
a) a flavin-dependent enzyme that has a flavin bound to it and that has specificity for the analyte, b) a tetrazolium dye precursor, c) an electron transfer agent, and d) a nitrite salt.
Specification