Human liver cell line

• US 7,015,036 B2
• Filed: 09/25/2001
• Issued: 03/21/2006
• Est. Priority Date: 09/25/2000
• Status: Active Grant

• Current Assignee: United States Army (U.S. Department Of Defense)
• Original Assignee: The United States Of America As Represented By The Secretary Of The Army
• Inventors: Prachumsri, Jetsumon, Yimamnuaychok, Nongnuch
• Primary Examiner(s): MINNIFIELD, NITA M
• Application Number:
  US09/962,364
• Publication Number:
  US 20020045262A1
• Time in Patent Office: 1,638 Days
• Field of Search: 435/91.1, 435/370, 435/258.2, 435/325, 435/366, 435/373, 435/374, 435/383, 435/378
• US Class Current: 435/370
• CPC Class Codes: C12N 2500/36 Lipids
• CPC Class Codes: C12N 2500/38 Vitamins
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/33 Insulin together with transferrin C12N2500/25; Insulin-like growth factors C12N2501/105
• CPC Class Codes: C12N 2501/335 Glucagon; Glucagon-like peptide [GLP]; Exendin
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 2503/02 Drug screening
• CPC Class Codes: C12N 2510/02 Cells for production
• CPC Class Codes: C12N 2510/04 Immortalised cells
• CPC Class Codes: C12N 5/067 Hepatocytes
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

In this application is described the establishment and maintanence of a normal human hepatocyte cell line able to support complete development of malaria parasite development in vitro. Advantages and uses of the cell line are also described.

Claims

1. A continually proliferating cell line established from isolated normal human liver tissue wherein the cell line is not established on a precoated growth surface.

2. The cell line of claim 1 having the ability to support growth of a liver stage malaria parasite.

3. The cell line of claim 2 wherein said malaria parasite is chosen from the group P. falciparum and P. vivax.

5. A method for developing liver stage malaria parasites in vitro, said method comprising infecting the cell line of claim 1 with sporozoites of said parasites.

6. A method for developing liver stage malaria parasites in vitro, said method comprising infecting the cell line of claim 1 with sporozoites of said parasites;

and incubating the infected cell line in the presence of erythrocytes.

7. The method of claim 5 wherein said cell line is HC-04.

8. The method of claim 6 wherein said cell line is HC-04.

9. The method of claim 6 wherein said parasite is chosen from the group consisting of P. falciparum and P. vivax.

10. The method of claim 5 wherein said parasite is chosen from the group consisting of P. falciparum and P. vivax.

11. The method of claim 7 wherein said parasite is chosen from the group consisting of P. falciparum and P. vivax.

12. The method of claim 8 wherein said parasite is chosen from the group consisting of P. falciparum and P. vivax.

4. A continually proliferating cell line established from isolated normal human liver tissue, wherein said cell line is HC-04 having ATCC accession number PTA-3441.