Multi-array, multi-specific electrochemiluminescence testing
First Claim
Patent Images
1. A method for conducting an electrochemiluminescence binding assay for detecting or measuring an analyte of interest in a sample comprising:
- (a) contacting an assay electrode with a sample and a component of said assay linked to an electrochemiluminescent label, said assay electrode;
(i) comprising a composite containing a polymer matrix and a multiplicity of carbon particles dispersed therein; and
(ii) having a binding domain containing a binding reagent, wherein said binding reagent is immobilized on said electrode;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said binding domain in the presence of a reaction medium suitable for conducting an electrochemiluminescence assay; and
(c) detecting or measuring electrochemiluminescence from said binding domain;
wherein said detected or measured electrochemiluminescence correlates to the presence or amount of said analyte in said sample.
0 Assignments
0 Petitions
Accused Products
Abstract
Materials and methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use multiply specific testing procedures.
186 Citations
29 Claims
-
1. A method for conducting an electrochemiluminescence binding assay for detecting or measuring an analyte of interest in a sample comprising:
-
(a) contacting an assay electrode with a sample and a component of said assay linked to an electrochemiluminescent label, said assay electrode; (i) comprising a composite containing a polymer matrix and a multiplicity of carbon particles dispersed therein; and (ii) having a binding domain containing a binding reagent, wherein said binding reagent is immobilized on said electrode; (b) applying a voltage waveform effective to trigger electrochemiluminescence at said binding domain in the presence of a reaction medium suitable for conducting an electrochemiluminescence assay; and (c) detecting or measuring electrochemiluminescence from said binding domain; wherein said detected or measured electrochemiluminescence correlates to the presence or amount of said analyte in said sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
-
-
27. A method for conducting electrochemiluminescence binding assays for detecting or measuring a plurality of analytes of interest in a sample comprising:
-
(a) contacting an assay electrode with a sample and one or more assay components linked to electrochemiluminescent labels, said assay electrode; (i) comprising a composite containing a polymer matrix and a multiplicity of carbon particles dispersed therein; and (ii) having a plurality of binding domains containing binding reagents, wherein said binding reagents are immobilized on said electrode; (b) applying a voltage waveform effective to trigger electrochemiluminescence at said binding domains in the presence of a reaction medium suitable for conducting an electrochemiluminescence assay; and (c) detecting or measuring electrochemiluminescence from said binding domains; wherein said detected or measured electrochemiluminescence at said binding domains correlates to the presence or amount of said analytes in said sample. - View Dependent Claims (28, 29)
-
Specification