Polynucleotides for the detection and quantification of hepatitis B virus nucleic acids
First Claim
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1. A composition comprising a forward primer and a reverse primer, wherein the forward primer consists of SEQ ID NO:
- 1 and the reverse primer consists of SEQ ID NO;
3, and wherein the primers can be used to detect hepatitis B virus (HBV).
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Abstract
The present invention provides polynucleotides that can specifically hybridise to Hepatitis B virus (HBV) nucleic acids from all genotypes. These polynucleotides can be used in genotype-independent detection and quantitation of HBV nucleic acids. For example, the polynucleotides can be used as primers and/or probes in amplification-based assays for either end-point detection or real-time monitoring of HBV nucleic acids in a test sample. The polynucleotides can additionally be provided as part of a kit for the detection and quantitation of HBV nucleic acids.
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7 Claims
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1. A composition comprising a forward primer and a reverse primer, wherein the forward primer consists of SEQ ID NO:
- 1 and the reverse primer consists of SEQ ID NO;
3, and wherein the primers can be used to detect hepatitis B virus (HBV). - View Dependent Claims (2, 3, 7)
- 1 and the reverse primer consists of SEQ ID NO;
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4. A kit for the amplification of nucleic acid sequences comprising a forward primer and a reverse primer, wherein the forward primer consists of SEQ ID NO:
- 1 and the reverse primer consists of SEQ ID NO;
3, and wherein the primers can be used to detect hepatitis B virus (HBV). - View Dependent Claims (5, 6)
- 1 and the reverse primer consists of SEQ ID NO;
Specification