Polynucleotides for the detection and quantification of hepatitis B virus nucleic acids

• US 7,015,317 B2
• Filed: 05/02/2002
• Issued: 03/21/2006
• Est. Priority Date: 05/02/2002
• Status: Expired due to Term

• Current Assignee: Abbott Molecular Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Rhoads, James, Mullen, Carolyn
• Primary Examiner(s): Parkin, Jeffrey S.
• Assistant Examiner(s): Le, Emily M.
• Application Number:
  US10/138,184
• Publication Number:
  US 20050164353A1
• Time in Patent Office: 1,419 Days
• Field of Search: 530/300, 930/10, 930/220, 930/223, 435 5- 6, 435/4, 435/29, 435/34, 435/39, 536/24.3, 536/24.33
• US Class Current: 536/24.3
• CPC Class Codes: C12Q 1/706 for hepatitis

Abstract

The present invention provides polynucleotides that can specifically hybridise to Hepatitis B virus (HBV) nucleic acids from all genotypes. These polynucleotides can be used in genotype-independent detection and quantitation of HBV nucleic acids. For example, the polynucleotides can be used as primers and/or probes in amplification-based assays for either end-point detection or real-time monitoring of HBV nucleic acids in a test sample. The polynucleotides can additionally be provided as part of a kit for the detection and quantitation of HBV nucleic acids.

Claims

1. A composition comprising a forward primer and a reverse primer, wherein the forward primer consists of SEQ ID NO:

1 and the reverse primer consists of SEQ ID NO;

3, and wherein the primers can be used to detect hepatitis B virus (HBV).

2. The composition of claim 1 further comprising an isolated nucleic acid sequence selected from the group consisting of SEQ ID NO:

2, SEQ ID NO;

17, SEQ ID NO;

21, SEQ ID NO;

26 and SEQ ID NO;

28.

3. The composition of claim 1 further comprising an isolated nucleic acid sequence as set forth in SEQ ID NO:

27.

7. A method for detecting Hepatitis B virus (HBV) in a test sample suspected of containing or known to contain one or more target HBV nucleic acid sequences comprising:

a) contacting said test sample with the forward and reverse primers of claim 1 under hybridizing conditions;

b) amplifying nucleic acid sequence or sequences that may be present in the test sample; and

c) detecting the amplification of one or more target HBV nucleic acid sequences, wherein a positive detection of a target HBV nucleic acid sequence is indicative of the presence of HBV in the test sample.

4. A kit for the amplification of nucleic acid sequences comprising a forward primer and a reverse primer, wherein the forward primer consists of SEQ ID NO:

1 and the reverse primer consists of SEQ ID NO;

3, and wherein the primers can be used to detect hepatitis B virus (HBV).

5. A kit for the amplification of nucleic acid sequences of claim 4 further comprising an isolated nucleic acid sequence selected from the group consisting of SEQ ID NO:

2, SEQ ID NO;

17, SEQ ID NO;

21, SEQ ID NO;

26 and SEQ ID NO;

28.

6. A kit for the amplification of nucleic acid sequences of claim 4 further comprising an isolated nucleic acid sequence as set forth in SEQ ID NO:

27.