Method for measuring concentration of component contained in bodily fluid and apparatus for measuring concentration of component contained in bodily fluid

• US 7,016,021 B2
• Filed: 05/19/2003
• Issued: 03/21/2006
• Est. Priority Date: 06/19/2002
• Status: Expired due to Fees

• Current Assignee: Omron Healthcare Company Limited (Omron Corporation)
• Original Assignee: Omron Corporation
• Inventors: Nakajima, Satoshi, Sakoda, Yusaku, Tokita, Muneo
• Primary Examiner(s): STAFIRA, MICHAEL PATRICK
• Application Number:
  US10/440,423
• Publication Number:
  US 20040012788A1
• Time in Patent Office: 1,037 Days
• Field of Search: 356 39- 42, 356128-137, 356335-343, 356445-448, 600309-310, 600316-324, 600345-352, 600/365
• US Class Current: 356/39
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14558 by polarisation
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658

Abstract

A measuring unit is placed at the surface of a living body. The pressure inside of a glass container is reduced by a pressure reducing tank, so that a subcutaneous exuding fluid is taken out at the surface of the living body from under a skin. The subcutaneous exuding fluid is allowed as a sample to be measured to reach a metallic film through porous molecular weight selective films. A light beam emitted from a light source passes through a prism, and then, is reflected on the metallic film, and finally, is received on a linear array sensor. A minimum intensity position on the linear array sensor, generated by surface plasmon resonance is obtained, and then, a resonant angle is detected. The concentration of substance to be measured contained in the sample to be measured is calculated based on the resonant angle and position. Thus, the concentration of a component contained in a bodily fluid can be readily measured for a long time of period in a continuous manner without any special treatment by taking a very small quantity of exuding fluid without any pain.

Claims

1. A method for measuring the concentration of a component contained in a bodily fluid comprising the steps of:

taking a bodily fluid from a living body;

separating a sample to be measured from said bodily fluid taken in said bodily fluid taking step;

measuring the refractive index of said sample separated in said sample separating step; and

calculating the concentration of substance to be measured contained in said sample to be measured or said bodily fluid based on said refractive index measured in said refractive index measuring step,

wherein a porous molecular weight selective film for allowing the substance contained in said bodily fluid to selectively permeate therethrough is used in said sample separating step.

2. A method for measuring the concentration of a component contained in a bodily fluid as claimed in claim 1, wherein said bodily fluid is selected from the group consisting of a subcutaneous exuding fluid, sweat, a gingival crevice fluid, blood and urine.

3. A method for measuring the concentration of a component contained in a bodily fluid as claimed in claim 1, wherein either one or both of pressure reduction and reverse iontophoresis are used in said bodily fluid taking step.

4. A method for measuring the concentration of a component contained in a bodily fluid as claimed in claim 1, wherein said porous molecular weight selective film is a laminated film in which a plurality of films different in selectiveness from each other are laminated one on another.

5. A method for measuring the concentration of a component contained in a bodily fluid as claimed in claim 1 or claim 4, wherein said porous molecular weight selective film is one film or a plurality of films selected from the group consisting of a fluororesin film, a cellulose-based film, an ion complex film, an albumin fixing film and an etching fine pore treatment polycarbonate film.

6. A method for measuring the concentration of a component contained in a bodily fluid as claimed in claim 1, wherein surface plasmon resonance is used in said refractive index measuring step.

7. A method for measuring the concentration of a component contained in a bodily fluid as claimed in claim 1, wherein the substance to be measured contained in said sample or said bodily fluid is either one or both of glucose and protein.

8. An apparatus for measuring the concentration of a component contained in a bodily fluid comprising:

a light source unit for measurement including at least a light source;

a porous molecular weight selective film which is brought into close contact with the surface of a living body;

a bodily fluid taking unit for allowing a bodily fluid to be exuded from said surface of the living body, and then, to permeate through said porous molecular weight selective film, thus obtaining a sample to be measured;

an optical element for allowing light emitted from said light source unit for measurement to enter into said sample to be measured on said porous molecular weight selective film, and further, for reflecting said light on said sample to be measured;

a light receiving element for receiving said light reflected on said sample to be measured, emitted from said optical element;

a control unit including a concentration calculator for calculating the concentration of substance to be measured contained in said sample or said bodily fluid in response to a signal output from said light receiving element; and

a display unit for displaying said concentration obtained by said concentration calculator.

9. An apparatus for measuring the concentration of a component contained in a bodily fluid as claimed in claim 8, wherein said optical element is a surface plasmon element having a metallic film at a surface opposite to said porous molecular weight selective film.

10. An apparatus for measuring the concentration of a component contained in a bodily fluid as claimed in claim 8 or claim 9, wherein said light source is a laser.

11. An apparatus for measuring the concentration of a component contained in a bodily fluid as claimed in claim 8 or claim 9, wherein said light source is an LED.

12. An apparatus for measuring the concentration of a component contained in a bodily fluid as claimed in claim 9, wherein said light source unit for measurement includes a polarizing plate for polarizing the light emitted from said light source.

13. An apparatus for measuring the concentration of a component contained in a bodily fluid as claimed in claim 9, wherein said concentration calculator calculates a resonant angle and a position in response to the signal sent from said light receiving element, so as to calculate said concentration of the substance to be measured.

14. An apparatus for measuring the concentration of a component contained in a bodily fluid as claimed in claim 8 or claim 9, wherein said porous molecular weight selective film, or said porous molecular weight selective film and said optical element can be separated from the main body of the apparatus for measuring the concentration of a component contained in a bodily fluid.

15. An apparatus for measuring the concentration of a component contained in a bodily fluid as claimed in claim 8 or claim 9, further comprising a case for covering said light source unit for measurement, said porous molecular weight selective film, said optical element and said light receiving element, wherein a part of said case and said porous molecular weight selective film are transparent, so that exudation of said bodily fluid from said surface of the living body or the state in which said bodily fluid permeates through said porous molecular weight selective film can be visually checked.

16. An apparatus for measuring the concentration of a component contained in a bodily fluid as claimed in claim 9, wherein said light source unit for measurement includes a light source for emitting parallel light beams, incident angle varying means for varying the incident angle of said parallel light beam, a focusing lens and a linear array.

17. An apparatus for measuring the concentration of a component contained in a bodily fluid as claimed in claim 9, wherein said light source unit for measurement includes a light source for emitting parallel light beams, wavelength varying means for varying the wavelength of said parallel light beam and a focusing lens.

18. An apparatus for measuring the concentration of a component contained in a bodily fluid as claimed in claim 9, wherein said bodily fluid taking unit adopts reverse iontophoresis, and said metallic film of the surface plasmon element and one of electrodes for the reverse iontophoresis are used commonly with each other.