Detection of analytes using reorganization energy

• US 7,018,523 B2
• Filed: 04/24/2001
• Issued: 03/28/2006
• Est. Priority Date: 06/12/1997
• Status: Expired due to Term

• Current Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Original Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Inventors: Meade, Thomas J.
• Primary Examiner(s): Monshipouri, Maryam
• Application Number:
  US09/841,809
• Publication Number:
  US 20020033345A1
• Time in Patent Office: 1,799 Days
• Field of Search: 205/778, 205/777.5, 205/793.5, 204/418, 204/415, 204/290, 204/403, 435/4, 435/5, 435/6, 435/7.1, 435/7.71, 435/7.72, 435/7.8, 435/7.9, 435/7.91, 435/7.93
• US Class Current: 205/777.5
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

The invention relates to novel methods and compositions for the detection of analytes using the nuclear reorganization energy, λ, of an electron transfer process.

Claims

1. A composition comprising an array of electrodes, each electrode comprising a covalently attached binding ligand and a covalently attached solvent accessible transition metal complex comprising a metal selected from the group consisting of manganese, technetium, rhenium, iron, ruthenium, osmium, cobalt, rhodium, iridium, nickel, palladium, platinum, copper, silver and gold.

2. A composition according to claim 1 wherein said solvent accessible transition metal complex has at least two coordination sites occupied by polar coordination groups.

3. A composition according to claim 1 wherein said solvent accessible transition metal complex has at least one coordination site occupied by a water molecule.

4. A composition according to claim 1 wherein said electrode further comprises a self-assembled monolayer.

5. A composition according to claim 1 wherein said solvent accessible transition metal complex is covalently attached to said electrode via a spacer.

6. A composition according to claim 5 wherein said spacer is a conductive oligomer.

7. A composition according to claim 1 wherein said solvent accessible transition metal complex is linked to said binding ligand to form a redox active complex.

8. A composition according to claim 1 wherein said binding ligand is covalently attached to said electrode via a conductive oligomer.

9. A composition according to claim 1 wherein said binding ligand will bind a protein.

10. A composition according to claim 1 wherein said binding ligand will bind a nucleic acid.

11. A composition according to claim 1 wherein said binding ligand is a protein.

12. A composition according to claim 1 wherein said solvent accessible transition metal complex is attached to said binding ligand.

13. A composition according to claim 6 wherein said conductive oligomer has the formula:

wherein Y is an aromatic group;

n is an integer from 1 to 50;

g is either 1 or zero;

e is an integer from zero to 10; and

m is zero or 1;

wherein when g is 1, B—

D is a conjugated bond; and

wherein when g is zero, e is 1 and D is preferably carbonyl, or a heteroatom moiety, wherein the heteroatom is selected from the group consisting of oxygen, sulfur, nitrogen and phosphorus.

14. A composition according to claim 13 wherein said aromatic group is phenyl, g is 1, and B—

D is an acetylene linkage.

15. A composition according to claim 6 wherein said conductive oligomer has the formula:

wherein

n is an integer from 1 to 50;

m is zero or 1;

C is carbon;

J is carbonyl or a heteroatom moiety, wherein the heteroatom is selected from the group consisting of oxygen, nitrogen, silicon, phosphorus and sulfur; and

G is bond selected from alkane, alkene or acetylene.

16. A composition according to claim 1 wherein said metal is gold.