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Near infrared chemical imaging microscope

  • US 7,019,296 B2
  • Filed: 10/24/2005
  • Issued: 03/28/2006
  • Est. Priority Date: 10/13/2000
  • Status: Expired due to Fees
First Claim
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1. A method for producing a volumetric image of a sample comprising the steps of:

  • A) providing a near infrared imaging detection system and a visible imagery system, wherein said near infrared imaging detection system comprises;

    i) an illumination source for illuminating an area of a sample using light in the near infrared radiation wavelength;

    ii) a device for collecting a spectrum of near infrared wavelength radiation light transmitted, reflected, or emitted from said illuminated area of said sample and producing a collimated beam therefrom;

    iii) a near infrared imaging spectrometer for selecting a near infrared radiation image of said collimated beam; and

    iv) a detector for capturing said selected near infrared images;

    v) an algorithm for processing the near infrared images captured by the detector and visible image data captured from said area of said sample using said visible imagery system;

    B) moving said sample relative to an objective;

    C) collecting near infrared and visible images of said sample relative to said objective in a plurality of focus depths by repeating steps A–

    B; and

    D) processing said collected near infrared and visible images to reconstruct said volumetric image of said sample.

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