GigaMatrix holding tray having through-hole wells
First Claim
1. A plate which comprises:
- a base having a first surface and a second surface; and
a plurality of substantially parallel elongated capillary tubes supported by said base, wherein each said tube has an interior wall presenting an interior surface to surround and define a lumen extending through said base between said first surface and said second surface, and an outer wall surrounding said interior wall and said lumen for absorbing light directed toward said interior surface of said interior wall, and further wherein each said tube defines a longitudinal axis and has an aspect ratio greater than about 5;
1 with an inner diameter less than approximately five hundred microns, and further wherein each said tube acts to optically distinguish light directed from inside said lumen toward said interior surface thereof from light directed along said axis for optical detection of said tube.
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0 Petitions
Accused Products
Abstract
A GigaMatrix plate for holding a large number of small-volume fluid samples includes a base for supporting a plurality of substantially parallel, elongated capillary tubes. Each tube defines a lumen that extends through the base, and each lumen has an aspect ratio greater than about 5:1. Dimensionally, each lumen has an inner diameter that is less than approximately five hundred microns and it has a length greater than about five millimeters. Further, each tube acts to optically distinguish light that is directed toward it from the sample whenever the sample fluoresces inside the tube lumen. Also, however, light from the sample that is directed axially through the tube is emitted therefrom for optical detection of the tube and the sample therein.
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Citations
22 Claims
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1. A plate which comprises:
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a base having a first surface and a second surface; and
a plurality of substantially parallel elongated capillary tubes supported by said base, wherein each said tube has an interior wall presenting an interior surface to surround and define a lumen extending through said base between said first surface and said second surface, and an outer wall surrounding said interior wall and said lumen for absorbing light directed toward said interior surface of said interior wall, and further wherein each said tube defines a longitudinal axis and has an aspect ratio greater than about 5;
1 with an inner diameter less than approximately five hundred microns, and further wherein each said tube acts to optically distinguish light directed from inside said lumen toward said interior surface thereof from light directed along said axis for optical detection of said tube. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A plate for holding optically detectable samples which comprises:
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a base made of an interstitial material and having a first surface and a second surface, with said second surface being substantially parallel to said first surface, wherein said base supports a plurality of substantially parallel through-hole wells, with each said through-hole well defining a longitudinal axis and extending between said first surface and said second surface, and wherein said plurality of through-hole wells have a density greater than approximately two wells per square millimeter on said first and second surfaces of said base; and
a material surrounding and defining a lumen for each said through-hole well to optically distinguish light directed substantially away from said axis in said lumen from light directed substantially along said axis in said lumen for optical detection of said through-hole well. - View Dependent Claims (8, 9, 10)
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11. A method of preparing samples for optical detection which comprises the steps of:
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providing a plate comprising a base having a first surface and a second surface, and said plate has a plurality of substantially parallel, elongated capillary tubes supported by said base, wherein each said tube has an interior surface defining a lumen extending through said base between said first surface and said second surface, further wherein each said tube defines a longitudinal axis and has an aspect ratio greater than about 5;
1 with an inner diameter less than approximately five hundred microns, and further wherein each said tube acts to optically distinguish light directed from said sample inside said lumen toward said interior surface thereof, from light directed from said sample along said axis for optical detection of said tube and said sample therein;
immersing said first surface of said base into a container holding said samples in a liquid solution to wick said samples into said tubes by a capillary action; and
positioning a cap on at least one said surface of said base to reduce evaporation of said liquid solution. - View Dependent Claims (12, 13)
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14. A method for manufacturing a plate for holding a large number of small volume fluid samples which comprises the steps of:
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providing an elongated optical fiber having a first length, said optical fiber comprising a cylindrical shaped core glass surrounded by concentric layers of a sleeve glass and an interstitial material;
drawing down said optical fiber to a second length, said second length being longer than said first length;
cutting said drawn optical fiber into a plurality of sections;
stacking said sections to create a multi;
pressing a plurality of said multis together with said sections thereof substantially parallel to each other;
heating said plurality of multis to fuse said interstitial material between adjacent said sections to create an integral unit;
cutting said integral unit to establish a substantially same predetermined length for each said section; and
immersing said integral unit in acid to etch said core glass therefrom to create said plate with a base having a first surface and a second surface and a plurality of substantially parallel elongated capillary tubes supported by said base, wherein each said tube has an interior surface defining a lumen extending through said base between said first surface and said second surface, further wherein each said tube defines a longitudinal axis and has an aspect ratio greater than about 5;
1 with an inner diameter less than approximately five hundred microns, and further wherein each said tube acts to optically distinguish light directed from inside said lumen toward said interior surface thereof, from light directed along said axis for optical detection of said tube. - View Dependent Claims (15, 16, 17)
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18. A plate which comprises:
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a base having a first surface and a second surface; and
a plurality of substantially parallel elongated capillary tubes supported by said base, wherein each said tube has an interior surface defining a lumen extending through said base between said first surface and said second surface, further wherein each said tube defines a longitudinal axis and has an aspect ratio greater than about 5;
1 with an inner diameter less than approximately five hundred microns, and further wherein each said tube acts to optically distinguish light directed from inside said lumen toward said interior surface thereof from light directed along said axis for optical detection of said tube and wherein said tube is made of a sleeve glass, with said sleeve glass being heat treatable to make said tube opaque.
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19. A plate for holding optically detectable samples which comprises:
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a base having a first surface and a second surface, with said second surface being substantially parallel to said first surface, wherein said base supports a plurality of substantially parallel through-hole wells wherein each said through-hole well is a capillary tube having an aspect ratio greater than about 5;
1 with an inner diameter less than approximately five hundred microns, with each said through-hole well defining a longitudinal axis and extending between said first surface and said second surface, and wherein said plurality of through-hole wells have a density greater than approximately two wells per square millimeter on said first and second surfaces of said base; and
a material surrounding and defining a lumen for each said through-hole well to optically distinguish light directed substantially away from said axis in said lumen from light directed substantially along said axis in said lumen for optical detection of said through-hole well, and wherein said material of said through-hole well for each of said capillary tubes includes an interior wall surrounding and defining said lumen, and an outer wall surrounding said interior wall and said lumen. - View Dependent Claims (21, 22)
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20. A method of preparing samples for optical detection which comprises the steps of:
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providing a plate comprising a base having a first surface and a second surface, and said plate has a plurality of substantially parallel, elongated capillary tubes supported by said base, wherein each said tube has an interior surface defining a lumen extending through said base between said first surface and said second surface, further wherein each said tube defines a longitudinal axis and has an aspect ratio greater than about 5;
1 with an inner diameter less than approximately five hundred microns, and further wherein each said tube acts to optically distinguish light directed from said sample inside said lumen toward said interior surface thereof, from light directed from said sample along said axis for optical detection of said tube and said sample therein;
immersing said first surface of said base into a container holding said samples in a liquid solution to wick said samples into said tubes by a capillary action; and
placing a high vapor pressure fluid into said through-hole wells to protect said liquid solution from evaporation.
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Specification