Use of marrow-derived glial progenitor cells as gene delivery vehicles into the central nervous system
First Claim
1. A method of treating Parkinson'"'"'s disease, comprising:
- transfecting harvested bone marrow cells with a retroviral vector comprising a gene for glial cell line-derived neurotrophic factor (GDNF);
administering the transfected cells intravenously to a subject having Parkinson'"'"'s disease; and
allowing the transfected cells to migrate to the brain of the subject and express the GDNF gene, thereby treating the Parkinson'"'"'s disease.
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Abstract
The present disclosure relates to a method for introducing a hematopoietic cell into the brain of a mammal, by administering bone marrow-derived progenitor cells into the body of the mammal by intravenous injection. The bone marrow-derived cell is preferably a cell that differentiates into a glial cell.
The disclosure also relates to a method for delivery of therapeutic protein molecules into the brain of a mammal, by administering to a mammal an effective amount of bone marrow-derived progenitor cells which contain a gene having a nucleic acid sequence that encodes a functional therapeutic protein.
Isolated recombinant cells and a pharmaceutical composition are also provided.
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Citations
8 Claims
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1. A method of treating Parkinson'"'"'s disease, comprising:
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transfecting harvested bone marrow cells with a retroviral vector comprising a gene for glial cell line-derived neurotrophic factor (GDNF); administering the transfected cells intravenously to a subject having Parkinson'"'"'s disease; and allowing the transfected cells to migrate to the brain of the subject and express the GDNF gene, thereby treating the Parkinson'"'"'s disease. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method of treating Parkinson'"'"'s disease, comprising:
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culturing harvested bone marrow cells in a cell culture medium comprising IL-3, IL-6, and stem cell factor; transfecting the harvested bone marrow cells with a Moloney murine leukemia virus vector comprising a gene for glial cell line-derived neurotrophic factor (GDNF); administering the transfected cells intravenously to a subject having Parkinson'"'"'s disease; and allowing the transfected cells to migrate to the brain of the subject and express the GDNF gene, thereby treating the Parkinson'"'"'s disease.
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Specification