Method for the simultaneous and direct determination of serum cholesterol in high and low density lipoproteins using infrared spectroscopy

• US 7,022,527 B2
• Filed: 09/11/2002
• Issued: 04/04/2006
• Est. Priority Date: 09/12/2001
• Status: Active Grant

• Current Assignee: National Research Council Canada
• Original Assignee: National Research Council Canada
• Inventors: Mantsch, Henry H., Shaw, Anthony, Liu, Kan-Zhi
• Primary Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US10/485,413
• Publication Number:
  US 20040248309A1
• Time in Patent Office: 1,301 Days
• Field of Search: 436/63, 436/71, 436/164, 436/171, 356/51
• US Class Current: 436/71
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/359 using near infrared light
• CPC Class Codes: G01N 2201/1293 resolving multicomponent spectra
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: G01N 33/92 involving lipids, e.g. cholesterol , lipoproteins, or their receptors steroid hormones G01N33/743

Abstract

A method of simultaneously determining the concentrations of cardiovascular risk markers selected from the group consisting of High Density Lipoprotein cholesterol (HDL-C), Low Density Lipoprotein cholesterol (LDL-C), total cholesterol, triglycerides and oxidized LDL using infra-red and/or near infrared light is described.

Claims

1. A method of determining the concentration of High Density Lipoprotein cholesterol (HDL-C) comprising:

subjecting a serum sample to infra-red light;

collecting at least one spectrum of infrared light after interaction with the sample; and

determining the concentration of HDL-C in the sample based on the intensity of spectral features or patterns over at least one spectral range selected from the group consisting of 900–

1500 cm−

1, 1500–

1800 cm−

1, and 2800–

3500 cm−

1.

2. The method according to claim 1 wherein the concentration of HDL-C is derived from the spectra using a quantitation algorithm.

3. The method according to claim 2 wherein the quantitation algorithm is partial least squares analysis.

4. A method of determining the concentration of Low Density Lipoprotein cholesterol (LDL-C) comprising:

subjecting a serum sample to infra-red light;

collecting at least one spectrum of infrared light after interaction with the sample;

determining the concentration of LDL-C in the sample based upon the intensity of spectral features or patterns over at least one spectral range selected from the group consisting of 1700–

1800 cm−

1, and 2800–

3000 cm−

1.

5. The method according to claim 4 wherein the concentration of LDL-C is derived from the spectra using a quantitation algorithm.

6. The method according to claim 5 wherein the quantitation algorithm is partial least squares analysis.