Methods and systems for alignment of detection optics

• US 7,023,007 B2
• Filed: 01/16/2003
• Issued: 04/04/2006
• Est. Priority Date: 07/17/2001
• Status: Expired due to Term

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Inventors: Gallagher, Steven J.
• Primary Examiner(s): LE, QUE TAN
• Application Number:
  US10/345,699
• Publication Number:
  US 20030127610A1
• Time in Patent Office: 1,174 Days
• Field of Search: None
• US Class Current: 250/559.29
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G01B 11/14 for measuring distance or clearance between spaced objects or spaced apertures G01B11/26 takes precedence; rangefinders G01C3/00
• CPC Class Codes: G01B 11/26 for measuring angles or tapers; for testing the alignment of axes
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/058 Flat flow cell
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters

Abstract

The present invention provides methods and systems for aligning detection optics with a device by obtaining an optical profile of the system and comparing it with a preprogrammed layout of the various optical features of the device. The invention also provides methods of identifying devices by comparing the optical profile with a library of preprogrammed optical profiles of multiple devices.

Claims

1. A method of positioning an optical detection system with respect to a detection region of a microfluidic device, comprising:

a) positioning a light beam at a first position along a first axis and along a second axis of said device;

b) moving the light beam along the first axis from said first position to detect a first optical feature of the device;

c) moving the light beam along the first axis to detect a second optical feature of the device;

d) determining a first distance along the first axis between the first and second optical features of the device;

e) comparing the first distance to a preset distance between the first and second optical features of the device; and

f) moving the light beam to a second position along the second axis of said device if the measured distance is not equal to the preset distance between the first and second optical features.

2. The method of claim 1, wherein the first and second optical features comprise first and second microfluidic channels.

3. The method of claim 1, wherein said first axis comprises an x-axis of the device.

4. The method of claim 1 wherein said first axis comprises a y-axis of the device.

5. The method of claim 1, further comprising repeating steps (a) through (f) until the first distance is equal to the preset distance between the first and second optical features of the device.

6. The method of claim 1 wherein said detection region is located at the same position along the second axis at which the first distance between said first and second optical features is equal to the preset distance.

7. The method of claim 2, wherein the first and second microfluidic channels comprise a reaction channel and an alignment channel.

8. The method of claim 1, wherein the step of comparing the first distance to a preset distance further comprises calculating the position along the second axis at which the first distance is equal to the preset distance.

9. The method of claim 3, wherein the step of moving the light beam to a second position comprises moving the light beam along a y-axis of the device which is perpendicular to said first axis.

10. The method of claim 1, wherein detecting a first optical feature comprises detecting light reflected from a first optical feature, and detecting a second optical feature comprises detecting light reflected from a second optical feature.

11. The method of claim 1, wherein detecting a first optical feature comprises detecting light emitted from a first optical feature, and detecting a second optical feature comprises detecting light emitted from a second optical feature.

12. The method of claim 11, wherein the emission of light from the first and second optical features results from the fluorescence of a material contained within the first and second optical features.

13. The method of claim 1, wherein detecting a first optical feature comprises detecting a change in transmissivity or absorbance, and detecting a second optical feature comprises detecting a change in transmissivity or absorbance.

14. The method of claim 1 wherein said determining is performed by a computer.

15. The method of claim 14 wherein said computer stores the preset distance between the first and second optical features.

16. The method of claim 1 further comprising moving the light beam to a third optical feature that includes the detection region.

17. The method of claim 16 wherein the third optical feature comprises a microfluidic channel of the device.