Methods and systems for alignment of detection optics
First Claim
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1. A method of positioning an optical detection system with respect to a detection region of a microfluidic device, comprising:
- a) positioning a light beam at a first position along a first axis and along a second axis of said device;
b) moving the light beam along the first axis from said first position to detect a first optical feature of the device;
c) moving the light beam along the first axis to detect a second optical feature of the device;
d) determining a first distance along the first axis between the first and second optical features of the device;
e) comparing the first distance to a preset distance between the first and second optical features of the device; and
f) moving the light beam to a second position along the second axis of said device if the measured distance is not equal to the preset distance between the first and second optical features.
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Abstract
The present invention provides methods and systems for aligning detection optics with a device by obtaining an optical profile of the system and comparing it with a preprogrammed layout of the various optical features of the device. The invention also provides methods of identifying devices by comparing the optical profile with a library of preprogrammed optical profiles of multiple devices.
124 Citations
17 Claims
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1. A method of positioning an optical detection system with respect to a detection region of a microfluidic device, comprising:
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a) positioning a light beam at a first position along a first axis and along a second axis of said device; b) moving the light beam along the first axis from said first position to detect a first optical feature of the device; c) moving the light beam along the first axis to detect a second optical feature of the device; d) determining a first distance along the first axis between the first and second optical features of the device; e) comparing the first distance to a preset distance between the first and second optical features of the device; and f) moving the light beam to a second position along the second axis of said device if the measured distance is not equal to the preset distance between the first and second optical features. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification