Mammalian cell surface antigens; related reagents

• US 7,025,962 B1
• Filed: 04/10/2000
• Issued: 04/11/2006
• Est. Priority Date: 08/16/1996
• Status: Expired due to Fees

• Current Assignee: Merck Sharp & Dohme Corporation (Merck & Co., Inc.)
• Original Assignee: Schering Corporation (Merck & Co., Inc.)
• Inventors: Gorman, Daniel M., Zlotnik, Albert, Randall, Troy D.
• Primary Examiner(s): Schwadron, Ronald B.
• Application Number:
  US09/545,998
• Time in Patent Office: 2,192 Days
• Field of Search: 530/388.22, 530/388.7, 530/388.75, 530/388.85, 530/387.9, 530/389.6, 530/350, 530/391.1, 530/387.3, 530/391.3, 435/975, 424/133.1, 424/139.1, 424/143.1, 424/144.1, 424/154.1, 424/156.1, 424/173.1
• US Class Current: 424/133.1
• CPC Class Codes: A61K 2039/55516 Proteins; Peptides
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/39 characterised by the immunostimulating additives, e.g. chemical adjuvants
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C07K 14/70578 NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95 NGF-receptor C07K14/71, TNF-receptor C07K14/7151
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: C07K 16/2878 against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
• CPC Class Codes: C07K 2317/70 characterized by effect upon binding to a cell or to an antigen
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 435/975 Kit

Abstract

Purified genes encoding a T cell surface antigen from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this antigen are provided. Methods of using said reagents and diagnostic kits are also provided.

Claims

1. An antibody or antigen binding fragment thereof which specifically binds a protein or peptide of at least 8 amino acids of SEQ ID NO:

2 or 4,

wherein said antibody or antigen binding fragment thereof is specifically immunoreactive with the protein of SEQ ID NO;

2 or 4 and not with other proteins.

2. A sterile or buffered composition comprising the antibody of claim 1.

3. A labeled antibody or antigen binding fragment thereof, wherein the antibody or antigen binding fragment thereof of claim 1 is detectably labeled.

4. An antibody or antigen binding fragment thereof attached to a solid substrate, wherein said antibody or antigen binding fragment thereof of claim 1 is attached to said solid substrate.

5. The antibody or antigen binding fragment thereof of claim 1, wherein the antibody is a:

a) polyclonal antibody;

or

b) monoclonal antibody.

6. The antibody or antigen binding fragment thereof of claim 1, wherein the antigen binding fragment is a:

a) Fab fragment;

b) Fab′

fragment;

or

c) F(ab)₂ fragment;

d) Fv fragment.

7. The antibody or antigen binding fragment thereof of claim 1, wherein said antibody is a humanized antibody or an antigen binding fragment thereof.

8. A kit comprising

a) a detectably labeled antibody or antigen binding fragment thereof which specifically binds a protein or peptide of at least 8 amino acids of SEQ ID NO:

2 or 4,

wherein said antibody or antigen binding fragment thereof is specifically immunoreactive with the protein of SEQ ID NO;

2 or 4 and not with other proteins;

b) a protein or peptide of at least 8 amino acids of SEQ ID NO;

2 or 4 immobilized to a solid support; and

c) instructions for use.