Reading chemical arrays

• US 7,027,930 B2
• Filed: 01/31/2001
• Issued: 04/11/2006
• Est. Priority Date: 01/31/2001
• Status: Expired due to Fees

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Cattell, Herbert F.
• Primary Examiner(s): Moran, Marjorie A.
• Assistant Examiner(s): Clow, Lori A.
• Application Number:
  US09/775,163
• Publication Number:
  US 20020102558A1
• Time in Patent Office: 1,896 Days
• Field of Search: 702/19, 702/22, 382/282, 382/286, 382/305, 435/174
• US Class Current: 702/19
• CPC Class Codes: G16B 25/00 ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression

Abstract

Methods of using an addressable array of biopolymers which has been exposed to a sample, and apparatus and computer program products for use of the arrays. In one embodiment the method includes detecting signals from the exposed array to obtain a signal image of the array. The array signal image is saved in a memory. Based on the detected signals, a shape of each region in one or more sets of multiple regions on the array signal image is established. A definition of the shapes of the established regions of each set is saved in a memory. Each region of each set is processed according to a corresponding routine for that set.

Claims

1. A method of reading an addressable array of biopolymers on a substrate which array has been exposed to a sample, comprising:

(a) detecting signals from the exposed array to obtain a signal image of the array;

(b) establishing, based on the detected signals, a shape of each region in one or more sets of multiple regions on the array signal image, and displaying the established shapes, wherein the shape of regions of a set include an identified shape of any of an array feature, a background region, and an identified inlier or outlier region within an array feature or background region;

(c) processing each region of each set according to a region processing routine; and

(d) saving the displayed shapes in a first file and the processed results in a second file.

2. The method according to claim 1 wherein the biopolymers are polynucleotides.

3. The method according to claim 2 wherein the biopolymers are DNA.

4. The method according to claim 1 wherein the first file is smaller than the second file.

5. The method according to claim 4 wherein the shape of regions of a second set are established each of which comprises a local background region of lower signal strength than a corresponding detected feature about which the background region is positioned.

6. The method according to claim 5 wherein the first routine comprises subtracting local background region signal strength from the corresponding detected feature signal strength.

7. The method according to claim 1 additionally comprising, subsequent to processing of each region, retrieving the saved shapes of the established regions and displaying images of them.

8. The method according to claim 7 wherein the shape of regions of a first set are established which comprise regions of signal strength higher than a predetermined value representing detected array features, surrounded by regions of lower signal strength representing detected background.

9. The method according to claim 8 wherein the regions of the first set are processed by a first routine comprising a summation of signal strength within sub-regions of each first set region to provide a total signal strength for each first set region.

10. The method according to claim 9 wherein the array signal image is a digital signal image and the sub-regions are pixels or pixel blocks.

11. The method according to claim 1 additionally comprising, saving the array signal image, and subsequent to processing each region, retrieving the array signal image and saved shapes of the established regions and displaying overlaid images of them.

12. The method according to claim 11 wherein each displayed region is linked to processed results for that region.

13. The method according to claim 12 additionally comprising evaluating the results from the processing based on the displayed retrieved shapes.

14. The method according to claim 12 additionally comprising, based on the displayed retrieved shapes, altering a parameter used in the processing and re-processing a region using the altered parameter.

15. The method according to claim 11 additionally comprising re-processing a region in response to an alteration of one of the shapes of a set using the altered shape.

16. A computer program product, comprising:

a computer readable storage medium having a computer program stored thereon for performing, when loaded into a computer, the steps of;

(a) receiving signals from a biopolymer array which has been exposed to a sample, as a signal image of the array;

(b) saving the array signal image in a memory;

(c) establishing, based on the detected signals, a shape of each region in one or more sets of multiple regions on the array signal image, wherein the shape of regions of a set include an identified shape of any of an array feature, a background region, and an identified inlier or outlier region within an array feature or background region;

(d) saving a definition of the shapes of the established regions of each set in a memory; and

(e) processing each region of each set according to a region processing routine.

17. A method of reading an addressable array of biopolymers on a substrate which array has been exposed to a sample, comprising:

(a) detecting signals from the exposed array to obtain a signal image of the array;

(b) establishing, based on the detected signals, a shape of each region in one or more sets of multiple regions on the array signal image, and displaying the established shapes, wherein the shape of regions of a set include an identified shape of an inlier or outlier region within an array feature;

(c) processing each region of each set according to a region processing routine; and

(d) saving the displayed shapes in a first file and the processed results in a second file.

18. The method according to claim 17 wherein the biopolymers are polynucleotides.

19. The method according to claim 18 wherein the biopolymers are DNA.

20. The method according to claim 17 wherein the first file is smaller than the second file.

21. The method according to claim 17 additionally comprising, subsequent to processing of each region, retrieving the saved shapes of the established regions and displaying images of them.

22. The method according to claim 17 additionally comprising, saving the array signal image, and subsequent to processing each region, retrieving the array signal image and saved shapes of the established regions and displaying overlaid images of them.

23. The method according to claim 22 wherein each displayed region is linked to processed results for that region.

24. The method according to claim 23 additionally comprising evaluating the results from the processing based on the displayed retrieved shapes.

25. The method according to claim 23 additionally comprising, based on the displayed retrieved shapes, altering a parameter used in the processing and re-processing a region using the altered parameter.

26. The method according to claim 22 additionally comprising re-processing a region in response to an alteration of one of the shapes of a set using the altered shape.

27. A computer program product, comprising:

a computer readable storage medium having a computer program stored thereon for performing, when loaded into a computer, the steps of;

(a) receiving signals from a biopolymer array which has been exposed to a sample, as a signal image of the array;

(b) saving the array signal image in a memory;

(c) establishing, based on the detected signals, a shape of each region in one or more sets of multiple regions on the array signal image, wherein the shape of regions of a set include an identified shape of an identified inlier or outlier region within an army feature;

(d) saving a definition of the shapes of the established regions of each set in a memory; and

e) processing each region of each set according to a region processing routine.

28. An apparatus for reading an addressable array of biopolymers on a substrate which array has been exposed to a sample, comprising:

(a) a detector to detect signals from the exposed array to obtain a signal image of the array;

(b) a memory; and

(c) a processor which;

establishes, based on the detected signals, a shape of each region in one or more sets of multiple regions on the array signal image, wherein the shape of regions of a set include an identified shape of any of an array feature, a background region, and an identified inlier or outlier region within an array feature or background region;

saves a definition of the shapes of the established regions of each set in a memory;

processes each region of each set according to a region processing routine; and

saves the displayed shapes in a first file and the processed results in a second file.

29. The apparatus according to claim 28 wherein the biopolymers are polynucleotides.

30. The apparatus according to claim 29 wherein the regions of the first set are processed by a first routine comprising a summation of signal strength within sub-regions of each first set region to provide a total signal strength for each first set region.

31. The apparatus according to claim 28 wherein the processor, subsequent to processing of each region, retrieves the saved shapes of the established regions and displays images of them.

32. The apparatus according to claim 28 wherein the processor saves the array signal image in memory and, subsequent to processing each region, retrieves the array signal image and saved shapes of the established regions, and displays overlaid images of them.

33. The apparatus according to claim 32 wherein each displayed region is linked to processed results for that region.

34. The apparatus according to claim 32 wherein the processor, in response to a user input alteration of one of the shapes of a set, reprocesses that region with the altered shape according to the corresponding routine for that set.

35. The apparatus according to claim 28 wherein the shape of regions of a first set are established which comprise regions of signal strength higher than a predetermined value representing detected array features, surrounded by regions of lower signal strength representing detected background.

36. The apparatus according to claim 35 wherein the processor establishes a shape of regions of a second set each of which comprises a local background region of lower signal strength than a corresponding detected feature about which the background region is positioned.

37. The apparatus method according to claim 36 wherein the first routine comprises subtracting local background region signal strength from the corresponding detected feature signal strength.

38. An apparatus for reading an addressable array of biopolymers on a substrate which array has been exposed to a sample, comprising:

(a) a detector to detect signals from the exposed array to obtain a signal image of the array;

(b) a memory; and

(c) a processor which;

establishes, based on the detected signals, a shape of each region in one or more sets of multiple regions on the array signal image, wherein the shape of regions of a set include an identified shape of an identified inlier or outlier region within an array feature;

saves a definition of the shapes of the established regions of each Set in a memory;

processes each region of each set according to a region processing routine; and

saves the displayed shapes in a first file and the processed results in a second file.

39. The apparatus according to claim 38 wherein the biopolymers are polynucleotides.